Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ANALYSIS OF THE P53 GENE ALTERATIONS IN MOUSE EMBRYO FIBROBLAST CELL-LINE BALB 3T12 AND ITS DERIVATIVE 312
Autore:
MURALI D; KHAR A;
Indirizzi:
CTR CELLULAR & MOL BIOL,UPPAL RD HYDERABAD 500007 ANDHRA PRADESH INDIA CTR CELLULAR & MOL BIOL HYDERABAD 500007 ANDHRA PRADESH INDIA
Titolo Testata:
Journal of Biosciences
fascicolo: 2, volume: 23, anno: 1998,
pagine: 119 - 123
SICI:
0250-5991(1998)23:2<119:AOTPGA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
WILD-TYPE P53; CLONAL EXPANSION; MUTANT-CELLS; AMPLIFICATION;
Keywords:
P53 GENE; MUTATIONS; MOUSE FIBROBLASTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
D. Murali e A. Khar, "ANALYSIS OF THE P53 GENE ALTERATIONS IN MOUSE EMBRYO FIBROBLAST CELL-LINE BALB 3T12 AND ITS DERIVATIVE 312", Journal of Biosciences, 23(2), 1998, pp. 119-123

Abstract

We have analysed the status of the p53 gene in the mouse embryo fibroblast cell line Balb 3T12 (TD50 = 10(6)) and its transformed clonal derivative 312 (TD50 = 10(4)) with an aim to determine whether there exists a correlation between increased tumorigenicity and clonal expansion of cells bearing a mutation in the p53 gene. While Southern hybridizations did not show any obvious changes in the p53 gene organization in 3T12 and 312 cells, sequencing the p53 cDNA revealed that 3T12 is mutated at the amino acid residue 233 (Tyr --> Asp) whereas 312 is mutated at: the residue 132 (Cys --> Trp). Exploiting the altered RFLP pattern due to mutations, we identified that 3T12 contains p53 alleles that are different from the already identified mutant p53. On the basis of these observations, we conclude that 3T12 and 312 have evolved independently.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:47:43