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Titolo:
DEPOSITION-RELATED SITES K5 K12 IN HISTONE H4 ARE NOT REQUIRED FOR NUCLEOSOME DEPOSITION IN YEAST/
Autore:
MA XJ; WU JS; ALTHEIM BA; SCHULTZ MC; GRUNSTEIN M;
Indirizzi:
UNIV CALIF LOS ANGELES,INST MOL BIOL,611 CIRCLE DR E,RM 302 LOS ANGELES CA 90095 UNIV CALIF LOS ANGELES,INST MOL BIOL LOS ANGELES CA 90095 UNIV CALIF LOS ANGELES,SCH MED,DEPT BIOL CHEM LOS ANGELES CA 90024 UNIV ALBERTA,DEPT BIOCHEM EDMONTON AB T6G 2H7 CANADA
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 12, volume: 95, anno: 1998,
pagine: 6693 - 6698
SICI:
0027-8424(1998)95:12<6693:DSKKIH>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-REPLICATION INVITRO; SILENT MATING LOCI; NEWLY SYNTHESIZED HISTONES; ASSEMBLY FACTOR-I; SACCHAROMYCES-CEREVISIAE; CELL-CYCLE; ACETYLATION SITES; N-TERMINUS; CHROMATIN; ACETYLTRANSFERASE;
Keywords:
NUCLEOSOME ASSEMBLY; ACETYLATION; SACCHAROMYCES CEREVISIAE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
X.J. Ma et al., "DEPOSITION-RELATED SITES K5 K12 IN HISTONE H4 ARE NOT REQUIRED FOR NUCLEOSOME DEPOSITION IN YEAST/", Proceedings of the National Academy of Sciences of the United Statesof America, 95(12), 1998, pp. 6693-6698

Abstract

Histone H4 can be acetylated at N-terminal lysines K5, K8, K12, and K16, but newly synthesized H4 is diacetylated at K5/K12 in diverse organisms. This pattern is widely thought to be important for histone deposition onto replicating DNA. To investigate the importance of K5/K12 we have mutagenized these lysines in yeast and assayed for nucleosome assembly. Assaying was done in the absence of the histone H3 N terminus, which has functions redundant with those of H4 in histone deposition, Nucleosome assembly was assayed by three methods. Because nucleosomedepletion may be lethal,,ve examined cell viability. We also analyzednucleosome assembly in vivo and in vitro by examining plasmid superhelicity density in whole cells and supercoiling in yeast cell extracts. All three approaches demonstrate that mutagenizing K5 and K12 together does not prevent cell growth and histone deposition in vivo or in vitro. Therefore, K5/K12 cannot be required for nucleosome assembly in yeast, It is only when the first three sites of acetylation-K5, K8, andK12-are mutagenized simultaneously that lethality occurs and assemblyis most strongly decreased both irt vivo and in vitro. These data argue for the redundancy of sites K5, K8, and K12 in the deposition of yeast histone H4.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:24:42