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Titolo:
B-CELL LYMPHOMA INDUCTION BY AKV MURINE LEUKEMIA VIRUSES HARBORING ONE OR BOTH COPIES OF THE TANDEM REPEAT IN THE U3 ENHANCER
Autore:
LOVMAND J; SORENSEN AB; SCHMIDT J; OSTERGAARD M; LUZ A; PEDERSEN FS;
Indirizzi:
AARHUS UNIV,DEPT BIOL MOL & STRUCT,CF MOLLERS ALLE,BLDG 130 DK-8000 AARHUS C DENMARK AARHUS UNIV,DEPT BIOL MOL & STRUCT DK-8000 AARHUS C DENMARK AARHUS UNIV,DEPT MED MICROBIOL & IMMUNOL DK-8000 AARHUS C DENMARK GSF MUNICH,RES CTR ENVIRONM & HLTH,DEPT MOL VIROL D-85764 NEUHERBERG GERMANY GSF MUNICH,RES CTR ENVIRONM & HLTH,INST PATHOL D-85764 NEUHERBERG GERMANY
Titolo Testata:
Journal of virology
fascicolo: 7, volume: 72, anno: 1998,
pagine: 5745 - 5756
SICI:
0022-538X(1998)72:7<5745:BLIBAM>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG TERMINAL REPEAT; INBRED MOUSE STRAINS; I TRANSCRIPTIONAL ACTIVATORS; FOCUS-FORMING VIRUSES; E-MU-MYC; T-CELL; NUCLEOTIDE-SEQUENCE; GENE-EXPRESSION; TRANSGENIC MICE; CONGENIC MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
70
Recensione:
Indirizzi per estratti:
Citazione:
J. Lovmand et al., "B-CELL LYMPHOMA INDUCTION BY AKV MURINE LEUKEMIA VIRUSES HARBORING ONE OR BOTH COPIES OF THE TANDEM REPEAT IN THE U3 ENHANCER", Journal of virology, 72(7), 1998, pp. 5745-5756

Abstract

Akv is an endogenous, ecotropic murine leukemia virus (MuLV) of the AKR strain. It has served as a prototype nonpathogenic or weakly pathogenic reference virus for studies of closely related potent lymphomagenic viruses such as the T-lymphomagenic SL3-3, We here report that Akv and an Akv mutant (Akv1-99) with only one copy of the 99-bp transcriptional enhancer induce malignant lymphomas with nearly 100% incidence and mean latency periods of 12 months after injection into newborn NMRImice. Molecular analysis of tumor DNA showed that the majority of thetumors were of the B-cell type. Sequence analysis of proviral transcriptional enhancers in DNA of B-cell lymphomas revealed conservation ofthe enhancer sequence, as well as a lack of sequence duplications of the Akvl-99 variant, while the repeat copy number in Akv was subject to fluctuations. In support of a B-cell specificity of the Akv enhancer, a murine plasmacytoma cell line was found to sustain three-to fivefold-higher transient transcriptional activity upon the Akv and Akvl-99 enhancers than upon the enhancer of the T-lymphomagenic SL3-3 MuLV. Thus, the overall picture is that Akv MuLV possesses a B-lymphomagenic potential and that the second copy of the 99-bp sequence seems to be ofminor importance for this potential. However, in one animal the lymphomas induced by Akv1-99 were of the T-cell type. Among the 24 tumors analyzed only this one harbored a clonal proviral integration in the c-myc locus. This provirus had undergone a duplication of a 113-bp sequence of the enhancer region, partly overlapping with the 99-bp repeat of Akv, as well as a few single nucleotide alterations within and outside the repeats, Taken together with previous studies, our results suggest that T-versus B-lymphomagenic specificity of the enhancer is governed by more than one nucleotide difference and that alterations in binding sites for transcription factors of the AML1 and nuclear-factor-1 families may contribute to this specificity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 11:17:34