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Titolo:
TRANSCRIPTIONAL REGULATION OF ENDOTHELIAL NITRIC-OXIDE SYNTHASE BY LYSOPHOSPHATIDYLCHOLINE
Autore:
CIESLIK K; ZEMBOWICZ A; TANG JL; WU KK;
Indirizzi:
UNIV TEXAS,SCH MED,VASC BIOL RES CTR,6431 FANNIN,MSB 5-016 HOUSTON TX77030 UNIV TEXAS,SCH MED,VASC BIOL RES CTR HOUSTON TX 77030 UNIV TEXAS,SCH MED,DIV HEMATOL HOUSTON TX 77030 ACAD SINICA,INST BIOMED SCI TAIPEI 115 TAIWAN NATL TAIWAN UNIV,COLL MED,NATL TAIWAN UNIV HOSP,DEPT INTERNAL MED TAIPEI 100 TAIWAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 24, volume: 273, anno: 1998,
pagine: 14885 - 14890
SICI:
0021-9258(1998)273:24<14885:TROENS>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; MAMMALIAN-CELLS; GENE-EXPRESSION; CULTURED HUMAN; SP1; ACTIVATION; INDUCTION; GROWTH; MESSENGER; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
K. Cieslik et al., "TRANSCRIPTIONAL REGULATION OF ENDOTHELIAL NITRIC-OXIDE SYNTHASE BY LYSOPHOSPHATIDYLCHOLINE", The Journal of biological chemistry, 273(24), 1998, pp. 14885-14890

Abstract

We have shown that lysophosphatidylcholine (lyso-PC) increases endothelial nitric-oxide synthase (eNOS) expression at the transcriptional level (Zembowicz, A., Tang, J.-L., and Wu, K. K. (1995) J. Biol. Chem. 270, 17006-17010). To elucidate the mechanism by which lyso-PC increases the eNOS transcription, we identified Sp1 sites at -104 to -90 and PEA3 sites at -40 to -24 as being involved in lyso-PC-induced promoteractivity. Site-directed mutagenesis of Sp1 sites resulted in a markedreduction of basal and lyso-PC-induced activity whereas PEA3 site mutation abrogated response to lyso-PC. Band shift assays revealed that lyso-PC augmented Sp1 binding activity. Pretreatment of cells or nuclear extracts with okadaic acid reduced the Sp1 binding activity. Furthermore, okadaic acid treatment abrogated the lyso-PC induced promoter augmentation. Lyse-PC increased the nuclear extract protein phosphatase 2A (PP2A) activity, which was suppressed by okadaic acid treatment. These results suggest that lyso-PC up-regulates eNOS transcription by a PP2A-dependent increase in Sp1 binding activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 06:21:02