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Titolo:
CISPLATIN BINDING-SITES ON HUMAN ALBUMIN
Autore:
IVANOV AI; CHRISTODOULOU J; PARKINSON JA; BARNHAM KJ; TUCKER A; WOODROW J; SADLER PJ;
Indirizzi:
UNIV EDINBURGH,DEPT CHEM,W MAINS RD EDINBURGH EH9 3JJ MIDLOTHIAN SCOTLAND UNIV EDINBURGH,DEPT CHEM EDINBURGH EH9 3JJ MIDLOTHIAN SCOTLAND UNIV LONDON BIRKBECK COLL,DEPT CHEM LONDON WC1H 0PP ENGLAND DELTA BIOTECHNOL LTD NOTTINGHAM NG7 1FD ENGLAND
Titolo Testata:
The Journal of biological chemistry
fascicolo: 24, volume: 273, anno: 1998,
pagine: 14721 - 14730
SICI:
0021-9258(1998)273:24<14721:CBOHA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SERUM-ALBUMIN; NUCLEAR MAGNETIC-RESONANCE; HUMAN-BLOOD-PLASMA; METHIONINE COMPLEXES; SECONDARY STRUCTURE; PLATINUM COMPLEXES; NMR-SPECTROSCOPY; PROTEIN-BINDING; DRUG-BINDING; IN-VITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
81
Recensione:
Indirizzi per estratti:
Citazione:
A.I. Ivanov et al., "CISPLATIN BINDING-SITES ON HUMAN ALBUMIN", The Journal of biological chemistry, 273(24), 1998, pp. 14721-14730

Abstract

Reactions of cisplatin (cis-[PtCl2(NH3)(2)]) with albumin are thoughtto play an important role in the metabolism of this anticancer drug. They are investigated here via (i) labeling of cisplatin with N-15 anduse of two-dimensional H-1,N-15 NMR spectroscopy, (ii) comparison of natural human serum albumin with recombinant human albumin (higher homogeneity and SH content), (iii) chemical modification of Cys, Met, andHis residues, (iv) reactions of bound platinum with thiourea, and (v)gel filtration chromatography, In contrast to previous reports, it isshown that the major sulfur-containing binding site involves Met and not Cys-34, and also a N ligand, in the form of an S,N macrochelate. Additional monofunctional adducts involving other Met residues and Cys-34 are also observed. During the later stages of reactions of cisplatin with albumin, release of NH3 occurs due to the strong trans influence of Met sulfur, which weakens the Pt-NH3 bonds, and protein cross-linking is observed. The consequences of these findings for the biological activity of cisplatin-albumin complexes are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:27:19