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Titolo:
ISOLATION OF HUMAN PROMOTER REGIONS BY ALU REPEAT CONSENSUS-BASED POLYMERASE-CHAIN-REACTION
Autore:
JENDRASCHAK E; KAMINSKI WE;
Indirizzi:
UNIV REGENSBURG,DEPT CLIN CHEM,FRANZ JOSEF STRAUSS ALLEE 11 D-93042 REGENSBURG GERMANY UNIV WASHINGTON,SCH MED,DEPT PATHOL SEATTLE WA 98195
Titolo Testata:
Genomics
fascicolo: 1, volume: 50, anno: 1998,
pagine: 53 - 60
SICI:
0888-7543(1998)50:1<53:IOHPRB>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN MONONUCLEAR-CELLS; FACTOR MESSENGER-RNA; A(4) HYDROLASE GENE; HUMAN GENOME; REPEATED SEQUENCES; MOLECULAR-CLONING; RAPID ISOLATION; PCR; DNA; FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
E. Jendraschak e W.E. Kaminski, "ISOLATION OF HUMAN PROMOTER REGIONS BY ALU REPEAT CONSENSUS-BASED POLYMERASE-CHAIN-REACTION", Genomics, 50(1), 1998, pp. 53-60

Abstract

Knowledge of the promoter structure is critical for an understanding of the regulation of genes. We demonstrate by analysis of 405 human genes that human promoter regions are flanked by upstream Alu repeat elements, typically at a distance of 0.5-5 kb from their protein-coding areas. We identified common Alu repeat consensus sequences (ARC) among the different members of the Alu subfamilies that can be used as universal anchor sites for polymerase chain reaction (PCR) amplification. Utilizing ARC-specific primers and oligonucleotides specific for the 5'end of a selected target gene, we show that sequences spanning unknown human gene promoter regions can be directly amplified by PCR from genomic DNA. This novel technique, termed ARC-PCR, allowed us to characterize the proximal promoters of the human LTA(4) hydrolase and SPARC genes, each within 1 day. (C) 1998 Academic Press.

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Documento generato il 02/12/20 alle ore 16:26:55