Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PHYSICAL LINKAGE OF THE HUMAN GROWTH-HORMONE GENE FAMILY AND THE THYROID-HORMONE RECEPTOR INTERACTING PROTEIN-1 GENE ON CHROMOSOME-17
Autore:
SURABHI RM; BOSE S; KUSCHAK BC; CATTINI PA;
Indirizzi:
UNIV MANITOBA,DEPT PHYSIOL WINNIPEG MB R3E 3J7 CANADA UNIV MANITOBA,DEPT PHYSIOL WINNIPEG MB R3E 3J7 CANADA
Titolo Testata:
Gene
fascicolo: 1, volume: 212, anno: 1998,
pagine: 67 - 75
SICI:
0378-1119(1998)212:1<67:PLOTHG>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHORIONIC SOMATOMAMMOTROPIN; ENHANCER ACTIVITY; SKELETAL-MUSCLE; CELL-LINE; EXPRESSION; LOCUS; CHROMATIN; MECHANISM; PITUITARY; EVOLUTION;
Keywords:
P1 CLONE; GENE MAPPING; CELL-TYPE-SPECIFIC GENE; DOWNSTREAM GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Surabhi et al., "PHYSICAL LINKAGE OF THE HUMAN GROWTH-HORMONE GENE FAMILY AND THE THYROID-HORMONE RECEPTOR INTERACTING PROTEIN-1 GENE ON CHROMOSOME-17", Gene, 212(1), 1998, pp. 67-75

Abstract

A P1 cloned insert of about 85.5 kilobases (kb) was isolated, containing four members of the human growth hormone/chorionic somatomammotropin (GH/CS) gene family and the thyroid hormone receptor interacting protein (TRIP-1) gene. The presence of the CS-like, CS-A, GH-variant and, most downstream, CS-B gene was confirmed by DNA blotting and sequence analysis. The TRIP-1 gene was detected 40 kb downstream of the CS-B gene and in the reverse transcriptional orientation to all the GH/CS genes. The TRIP-1 gene is highly homologous to the SUG-1 gene in yeast and is evolutionarily conserved among several species. Based on the common location of the GH and TRIP-1 (or homologue) genes on the same chromosome in the human, pig and rat genomes, we suggest that these lociare physically linked. Previously, it was reported that a muscle-specific sodium channel (SCN4A) gene is located immediately upstream of the pituitary growth hormone (GH-N) gene, and is linked to the GH gene locus in both humans and rats. This suggests a further linkage between the SCN4A, GH and TRIP-1 loci. Also, deoxyribonuclease hypersensitive sites have been reported in and around these loci and were associated with an important locus control region for the GH/CS genes. Unlike theGH/CS genes, we show, using reverse transcriptase-polymerase chain reaction that the TRIP-1 gene is expressed ubiquitously and, through RNAblotting, as a 1.4-kb transcript. This implies an open and active chromatin structure. The possible effect of this structure on the adjacent human GH/CS gene locus is discussed. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 10:36:37