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Titolo:
CHROMATIN DECONDENSATION, PRONUCLEUS FORMATION, METAPHASE ENTRY AND CHROMOSOME COMPLEMENTS OF HUMAN SPERMATOZOA AFTER INTRACYTOPLASMIC SPERM INJECTION INTO HAMSTER OOCYTES
Autore:
GOUD PT; GOUD AP; RYBOUCHKIN AV; DESUTTER P; DHONT M;
Indirizzi:
DEPT OBSTET & GYNAECOL,INFERTIL CTR,DE PINTELAAN 185 B-9000 GHENT BELGIUM STATE UNIV GHENT HOSP,DEPT OBSTET & GYNAECOL B-9000 GHENT BELGIUM
Titolo Testata:
Human reproduction
fascicolo: 5, volume: 13, anno: 1998,
pagine: 1336 - 1345
SICI:
0268-1161(1998)13:5<1336:CDPFME>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; ONE-CELL EMBRYOS; GOLDEN-HAMSTER; FERTILIZING-CAPACITY; INVITRO DEVELOPMENT; MOUSE OOCYTES; BLASTOCYST STAGE; MAMMALIAN EGGS; 2-CELL EMBRYOS; NUCLEI;
Keywords:
ICSI; MALE PRONUCLEUS ASYNCHRONY; PARTHENOGENETIC ACTIVATION; SPERM KARYOTYPING; SPONTANEOUS ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
66
Recensione:
Indirizzi per estratti:
Citazione:
P.T. Goud et al., "CHROMATIN DECONDENSATION, PRONUCLEUS FORMATION, METAPHASE ENTRY AND CHROMOSOME COMPLEMENTS OF HUMAN SPERMATOZOA AFTER INTRACYTOPLASMIC SPERM INJECTION INTO HAMSTER OOCYTES", Human reproduction, 13(5), 1998, pp. 1336-1345

Abstract

Obtaining karyotypes from human spermatozoa after microinjection intoSyrian golden hamster oocytes is difficult and the hitherto reported results are unsatisfactory. This may be related to the injection and culture technique or to the high susceptibility of the hamster oocytes to undergo parthenogenetic activation or both. Therefore, we investigated the hamster oocyte-human sperm microinjection model using the following two approaches: (i) application of contemporary techniques for injection (touching the sperm tail) and culture (hamster embryo culturemedium, HECM-3, 10% CO2) and (ii) omission of Ca2+ from the injectionmedium. Thus, in the first series of experiments, 252 hamster oocyteswere injected with human spermatozoa, Among the 219 (87%) oocytes that survived the injection procedure, the mean percentages of male pronucleus formation [two pronuclei (2PN), two polar bodies (PB)], mitotic metaphase entry and sperm chromosome spreads were 41.4, 27.8 and 18.2%respectively. Analysis of the oocytes which failed to develop the male pronucleus following injection revealed that most of them had developed only the hamster female PN while the sperm nuclei were either intact or swollen (partially decondensed), indicating that failure of oocyte activation was not the likely reason for the failure of male PN formation in these oocytes, In the next series of experiments, sibling oocytes were alternately injected with spermatozoa suspended either in the regular (1.9 mM Ca2+) or Ca2+-free injection medium (experiment set2, n = 278), A significant improvement was noted in the mean percentages of oocytes,vith 2PN, 2PB, metaphase entry and sperm chromosome spreads in the Ca2+-free group versus the regular group (2PN, 2PB: 51 versus 36.6 %, metaphase entry: 36.3 versus 26.9 % and sperm chromosome spreads: 28 versus 20.4%; all P < 0.04), Thus, parthenogenetic activation appears to be one of the contributing factors for the failure of male PN formation after heterospecific hamster ICSI, From these experiments it can be concluded that application of the advanced injection andculture techniques and omission of Ca2+ from the injection medium arepromising for the routine application of the hamster oocyte microinjection for karyotyping of human spermatozoa with poor fertilizing capacity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 08:13:57