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Titolo:
MONOCLONAL-ANTIBODIES TO SUBCLASS-SPECIFIC ANTIGENIC DETERMINANTS ON EQUINE IMMUNOGLOBULIN GAMMA-CHAIN AND THEIR CHARACTERIZATION
Autore:
SHEORAN AS; LUNN DP; HOLMES MA;
Indirizzi:
UNIV CAMBRIDGE,DEPT CLIN VET MED,MADINGLEY RD CAMBRIDGE CB3 0DS ENGLAND UNIV CAMBRIDGE,DEPT CLIN VET MED CAMBRIDGE CB3 0DS ENGLAND UNIV WISCONSIN,SCH VET MED MADISON WI 53706
Titolo Testata:
Veterinary immunology and immunopathology
fascicolo: 2, volume: 62, anno: 1998,
pagine: 153 - 165
SICI:
0165-2427(1998)62:2<153:MTSADO>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Keywords:
HORSES; IMMUNGLOBULINS; ANTIGENS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
8
Recensione:
Indirizzi per estratti:
Citazione:
A.S. Sheoran et al., "MONOCLONAL-ANTIBODIES TO SUBCLASS-SPECIFIC ANTIGENIC DETERMINANTS ON EQUINE IMMUNOGLOBULIN GAMMA-CHAIN AND THEIR CHARACTERIZATION", Veterinary immunology and immunopathology, 62(2), 1998, pp. 153-165

Abstract

This paper describes the production of a panel of monoclonal antibodies (mAbs) identifying the four recognised equine IgG subisotypes IgG, IgGa, IgGb, IgGc and IgG(T). Pure preparations of the subisotypes for use in immunisations and testing were produced using a combination of gel filtration, salt precipitation, ion exchange chromatography and protein A and Protein G affinity chromatography. The specificity of mAbsfor the IgG subisotypes was confirmed using ELISA assays, by characterisation of affinity purified proteins recognised by the mAbs, and by Western blotting of equine serum proteins. The expression of equine IgG subisotypes by B cells was examined by flow cytometry using the panel of mAbs. (C) 1998 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 20:38:31