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Titolo:
LYSINE-RICH GAMMA-ZEINS ARE SECRETED IN TRANSGENIC ARABIDOPSIS PLANTS
Autore:
ALVAREZ I; GELI MI; PIMENTEL E; LUDEVID D; TORRENT M;
Indirizzi:
CSIC,CTR INVEST & DESENVOLUPAMENT,DEPT MOL GENET E-08034 BARCELONA SPAIN CSIC,CTR INVEST & DESENVOLUPAMENT,DEPT MOL GENET E-08034 BARCELONA SPAIN UNIV BASEL,BIOCTR,DEPT BIOCHEM CH-4056 BASEL SWITZERLAND CTR INGN GENET & BIOTECNOL CAMAGUEY CUBA
Titolo Testata:
Planta
fascicolo: 3, volume: 205, anno: 1998,
pagine: 420 - 427
SICI:
0032-0935(1998)205:3<420:LGASIT>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOPLASMIC-RETICULUM RETENTION; PROTEIN-BODY FORMATION; SEED STORAGE PROTEINS; CELL-WALL PROTEINS; MAYS-L ENDOSPERM; STRUCTURAL DOMAINS; MAIZE ENDOSPERM; GLUTELIN-2; TOBACCO; BODIES;
Keywords:
ARABIDOPSIS (PROTEIN SECRETION); LYSINE-RICH GAMMA-ZEIN; PROLAMIN (MAIZE); SECRETORY PATHWAY; TRANSGENIC ARABIDOPSIS; ZEIN TARGETING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
I. Alvarez et al., "LYSINE-RICH GAMMA-ZEINS ARE SECRETED IN TRANSGENIC ARABIDOPSIS PLANTS", Planta, 205(3), 1998, pp. 420-427

Abstract

We have previously shown that the maize (Zea mays L.) storage prolamine gamma-zein, accumulates in endoplasmic reticulum-derived protein bodies in transgenic plants of Arabidopsis thaliana (L.) ecotype R + P. The retention of gamma-zein in the endoplasmic reticulum was found to be mediated by structural features contained in the polypeptide, an N-terminal proline-rich and a C-terminal cysteine-rich domain which werenecessary for the correct retention and assembly of gamma-zein withinprotein bodies (M.I. Cell et al., 1994, Plant Cell 6: 1911-1922). In the present work we incorporated in the gamma-zein gene lysine-rich coding sequences which were positioned after the N-terminal proline-richdomain and at five amino-acid residues from the C-terminus. The targeting of lysine-rich gamma-zeins was analyzed by expression of chimericgenes regulated by the cauliflower mosaic virus (CaMV) 35S promoter in transgenic Arabidopsis plants. The lysine-rich gamma-zeins were detected by immunoblotting and we found that these proteins were modified posttranslationally to reach their mature form. Subcellular fractionation and immunocytochemical studies demonstrated that glycosylated lysine-rich gamma-zeins were secreted to the cell wall of transgenic Arabidopsis leaf cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:36:16