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Titolo:
SOLUTION STRUCTURE OF A NON-PALINDROMIC 16 BASE-PAIR DNA RELATED TO THE HIV-1 KAPPA-B SITE - EVIDENCE FOR BI-BII EQUILIBRIUM INDUCING A GLOBAL DYNAMIC CURVATURE OF THE DUPLEX
Autore:
TISNE C; HANTZ E; HARTMANN B; DELEPIERRE M;
Indirizzi:
INST PASTEUR,LAB RMN,CNRS URA 1129,28 RUE DR ROUX F-75015 PARIS FRANCE INST BIOL PHYSICOCHIM,LAB BIOCHIM THEOR,CNRS UPR 9080 F-75005 PARIS FRANCE
Titolo Testata:
Journal of Molecular Biology
fascicolo: 1, volume: 279, anno: 1998,
pagine: 127 - 142
SICI:
0022-2836(1998)279:1<127:SSOAN1>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; NUCLEAR-MAGNETIC-RESONANCE; RESTRAINED MOLECULAR-DYNAMICS; TRANSCRIPTION FACTOR; P50 HOMODIMER; BACKBONE CONFORMATION; FORCE-FIELD; NMR-SPECTRA; P-31 NMR; SPECTROSCOPY;
Keywords:
NMR; P-31 NMR; DNA-STRUCTURE; MOLECULAR MODELING; NF-KAPPA-B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
C. Tisne et al., "SOLUTION STRUCTURE OF A NON-PALINDROMIC 16 BASE-PAIR DNA RELATED TO THE HIV-1 KAPPA-B SITE - EVIDENCE FOR BI-BII EQUILIBRIUM INDUCING A GLOBAL DYNAMIC CURVATURE OF THE DUPLEX", Journal of Molecular Biology, 279(1), 1998, pp. 127-142

Abstract

H-1 and P-31 NMR spectroscopy have been used together with molecular modelling to determine the fine structure of a non-palindromic 16 bp DNA containing the NF-kappa B binding site. Much emphasis has been placed upon NMR optimization of both two-dimensional P-31 NMR techniques to extract structural information defining the phosphodiester backbone con formation and selective homonuclear 2D COSY experiments to determine sugar conformations. NMR data show evidence for a dynamic behaviourof steps flanking the ten base-pairs of the NF-kappa B binding site. A BI-BII equilibrium at these steps is demonstrated and two models foreach extreme conformation are proposed in agreement with NMR data. Lnthe refined BII structures, the NF-kappa B binding site exhibits an intrinsic curvature towards the major groove that is magnified by the four flanking steps in the BII conformation. Furthermore, the base-pairs are translated into the major groove. Thus, we present a novel mode of dynamic intrinsic curvature compatible with the DNA curvature observed in the X-ray structure of the p50-DNA complex. (C) 1998 Academic Press Limited.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 02:09:28