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Titolo:
A POSSIBLE MECHANISM UNDERLYING CORYMINE INHIBITION OF GLYCINE-INDUCED CL- CURRENT IN XENOPUS OOCYTES
Autore:
LEEWANICH P; TOHDA M; MATSUMOTO K; SUBHADHIRASAKUL S; TAKAYAMA H; AIMI N; WATANABE H;
Indirizzi:
TOYAMA MED & PHARMACEUT UNIV,WAKAN YAKU RES INST,DEPT PHARMACOL,2630 SUGITANI SUGITANI TOYAMA 93001 JAPAN TOYAMA MED & PHARMACEUT UNIV,WAKAN YAKU RES INST,DEPT PHARMACOL SUGITANI TOYAMA 93001 JAPAN PRINCE SONGKLA UNIV,FAC PHARMACEUT SCI,DEPT PHARMACEUT BOT & PHARMACOGNOSY SONGKHLA 90112 THAILAND CHIBA UNIV,FAC PHARMACEUT SCI CHIBA 263 JAPAN
Titolo Testata:
European journal of pharmacology
fascicolo: 2-3, volume: 348, anno: 1998,
pagine: 271 - 277
SICI:
0014-2999(1998)348:2-3<271:APMUCI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRAIN MESSENGER-RNA; GAMMA-AMINOBUTYRIC-ACID; RAT-BRAIN; HUNTERIA-ZEYLANICA; STRYCHNINE-BINDING; SPINAL-CORD; FUNCTIONAL EXPRESSION; HYPOTHALAMIC NEURONS; CHLORIDE CHANNELS; INDOLE ALKALOIDS;
Keywords:
XENOPUS OOCYTE; CL- CURRENT, GLYCINE-INDUCED; CORYMINE; STRYCHNINE; DIDS (4,4'-DIISOTHIOCYANOSTILBENE-2,2'-DISULFONIC ACID);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
P. Leewanich et al., "A POSSIBLE MECHANISM UNDERLYING CORYMINE INHIBITION OF GLYCINE-INDUCED CL- CURRENT IN XENOPUS OOCYTES", European journal of pharmacology, 348(2-3), 1998, pp. 271-277

Abstract

We previously reported that corymine, an alkaloid extracted from the leaves of Hunteria zeylanica native to Thailand, inhibited glycine-induced chloride current using a receptor expression model of Xenopus oocytes. In this study, we investigated the mechanism underlying the inhibitory action of this alkaloid on glycine current using the same model. Corymine inhibited glycine current in a noncompetitive fashion. Go-application with strychnine, a competitive glycine receptor antagonist,or 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), a Cl- channel blocker, corymine decreased the ED50 value of strychnine, but didnot change that of DIDS. Moreover, the inhibitory effects of corymineand either strychnine or DIDS were additive. The desensitization phase of glycine current showed two exponentials and corymine preferentially inhibited the fast component, whereas strychnine affected both of them to the same extent and DIDS preferentially inhibited the slow component. When these drugs were applied repeatedly, the inhibitory effects of corymine and strychnine were not use-dependent and reversible, while the effect of DIDS was use-dependent and irreversible. The inhibitory effect of corymine on gamma-aminobutyric acid (GABA) current was less potent than the effect on glycine current, while this alkaloid failed to affect acetylcholine and serotonin currents. These results demonstrate that corymine inhibits glycine-gated Ci-channels by interacting with the site different from that of DIDS. (C) 1998 Elsevier ScienceB.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 21:29:36