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Titolo:
PRODUCTION OF HUMAN LACTOFERRIN IN TRANSGENIC TOBACCO PLANTS
Autore:
SALMON V; LEGRAND D; SLOMIANNY MC; ELYAZIDI I; SPIK G; GRUBER V; BOURNAT P; OLAGNIER B; MISON D; THEISEN M; MEROT B;
Indirizzi:
UNIV SCI & TECH LILLE FLANDRES ARTOIS,CHIM BIOL LAB F-59655 VILLENEUVE DASCQ FRANCE UNIV SCI & TECH LILLE FLANDRES ARTOIS,CHIM BIOL LAB F-59655 VILLENEUVE DASCQ FRANCE UNIV SCI & TECH LILLE FLANDRES ARTOIS,CNRS,UNITE MIXTE RECH 111 F-59655 VILLENEUVE DASCQ FRANCE MERISTEM THERAPEUT F-63100 CLERMONT FERRA FRANCE
Titolo Testata:
Protein expression and purification
fascicolo: 1, volume: 13, anno: 1998,
pagine: 127 - 135
SICI:
1046-5928(1998)13:1<127:POHLIT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAS-LIQUID CHROMATOGRAPHY; LACTOTRANSFERRIN RECEPTOR; METHYL GLYCOSIDES; LINKED GLYCANS; CELL-LINES; IN-VITRO; IRON; EXPRESSION; GLYCOPROTEINS; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
V. Salmon et al., "PRODUCTION OF HUMAN LACTOFERRIN IN TRANSGENIC TOBACCO PLANTS", Protein expression and purification, 13(1), 1998, pp. 127-135

Abstract

Production and characterization of human lactoferrin (hLf) in transgenic tobacco is reported. We have engineered two constructs containing either the native signal peptide from human lactoferrin or the signal peptide from sweet potato sporamin fused to human lactoferrin encodingcDNA. N-terminal sequences of rhLf purified from tobacco were identical to Lf from human milk for both constructs. The tobacco rhLf presents a molecular mass closely identical to native protein. Overall sugar composition shows the presence of plant specific xylose while sialic acid is absent. Binding parameters of the recombinant molecule to both Jurkat lymphoblastic T-cells or HT29-18-C1 enterocytes are similar to those of human lactoferrin isolated from milk. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 18:20:38