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Titolo:
ENHANCEMENT OF BETA-AMYLOID PRECURSOR PROTEIN TRANSCRIPTION AND EXPRESSION BY THE SOLUBLE INTERLEUKIN-6 RECEPTOR INTERLEUKIN-6 COMPLEX
Autore:
RINGHEIM GE; SZCZEPANIK AM; PETKO W; BURGHER KL; ZHU SZ; CHAO CC;
Indirizzi:
HOECHST MARION ROUSSEL,NEUROSCI DIS GRP,ROUTE 202-206,POB 6800,ROOM L-219 BRIDGEWATER NJ 08807 MED RES FDN MINNEAPOLIS MN 55404
Titolo Testata:
Molecular brain research
fascicolo: 1, volume: 55, anno: 1998,
pagine: 35 - 44
SICI:
0169-328X(1998)55:1<35:EOBPPT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALZHEIMERS-DISEASE PATIENTS; MULTIPLE-SCLEROSIS LESIONS; MESSENGER-RNA EXPRESSION; RAT-BRAIN; TRANSGENIC MICE; IL-6 RECEPTOR; TNF-ALPHA; CHOLINERGIC NEURONS; HIPPOCAMPAL-NEURONS; MAJOR DEPRESSION;
Keywords:
ALZHEIMERS; BETA AMYLOID PRECURSOR PROTEIN; INTERLEUKIN-6; SOLUBLE INTERLEUKIN-6 RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
65
Recensione:
Indirizzi per estratti:
Citazione:
G.E. Ringheim et al., "ENHANCEMENT OF BETA-AMYLOID PRECURSOR PROTEIN TRANSCRIPTION AND EXPRESSION BY THE SOLUBLE INTERLEUKIN-6 RECEPTOR INTERLEUKIN-6 COMPLEX", Molecular brain research, 55(1), 1998, pp. 35-44

Abstract

We investigated a potential role for the soluble interleukin-6 receptor (slL-6R) in modulating interleukin-6 (IL-6) function in the centralnervous system by assessing IL-6 and slL-6R effects on beta-amyloid precursor protein (beta-APP) transcription and expression in cells of human neuronal origin. Cells transfected with a luciferase reporter plasmid containing a 3.8 kb DNA fragment of the beta-APP promoter were shown to have inducible promoter activity when treated with phorbol ester or basic fibroblast growth factor, but not when treated with lipopolysaccharide or IL-6, PCR amplification analysis revealed the presence of mRNA encoding the signaling subunit of the IL-6 receptor complex, the gp130 subunit, at levels approximating that found in human corticaltissue. The mRNA encoding the IL-6 receptor, however, was poorly expressed and was detectable only at high amplification cycles. When purified sIL-6R protein was added together with IL-6, there was a rapid induction of promoter activity within 2 h of stimulation followed by elevations in protein levels of both cell-associated and secreted beta-APP. Analysis of mRNA transcripts from human cortical brain tissue and cell cultures derived from fetal human brain demonstrated the presence of an alternatively spliced secreted form of the IL-6 receptor mRNA, suggesting that cells of the central nervous system may themselves be a source of sIL-6R protein. The capacity for sIL-6R to enhance IL-6 function and broaden the IL-6 target cell population in the brain has implications for the regulation of beta-APP expression in disease states such as Alzheimer's disease where elevations in brain IL-6 levels have been reported. (C) 1998 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 09:23:59