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Titolo:
IMPACT OF FIBROBLAST GROWTH FACTOR-2 ON TUMOR MICROVASCULAR ARCHITECTURE - A TRIDIMENSIONAL MORPHOMETRIC STUDY
Autore:
KONERDING MA; FAIT E; DIMITROPOULOU C; MALKUSCH W; FERRI C; GIAVAZZI R; COLTRINI D; PRESTA M;
Indirizzi:
JOHANNES GUTENBERG UNIV,INST ANAT,MAKROSKOP BEREICH,DEPT ANAT,BECHERWEG 13 D-55099 MAINZ GERMANY KONTRON ELEKT GMBH MUNICH GERMANY MARIO NEGRI INST PHARMACOL RES I-24100 BERGAMO ITALY UNIV BRESCIA,SCH MED,DEPT BIOCHEM SCI & BIOTECHNOL BRESCIA ITALY
Titolo Testata:
The American journal of pathology
fascicolo: 6, volume: 152, anno: 1998,
pagine: 1607 - 1616
SICI:
0002-9440(1998)152:6<1607:IOFGFO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR-PERMEABILITY FACTOR; IN-VIVO; FACTOR OVEREXPRESSION; EXTRACELLULAR-MATRIX; ENDOTHELIAL-CELLS; FACTOR FAMILY; BRAIN-TUMORS; ANGIOGENESIS; CANCER; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Konerding et al., "IMPACT OF FIBROBLAST GROWTH FACTOR-2 ON TUMOR MICROVASCULAR ARCHITECTURE - A TRIDIMENSIONAL MORPHOMETRIC STUDY", The American journal of pathology, 152(6), 1998, pp. 1607-1616

Abstract

Three cell clones originated by transfection of human endometrial adenocarcinoma HEC-1-B cells with fibroblast growth factor-2 (FGF-2) cDNAand characterized by a different capacity to produce and secrete the growth factor were transplanted subcutaneously in nude mice. Corrosioncasting of the tumor microvasculature of xenografts produced by injection of 2 x 10(6) or 10 x 10(6) FGF-2-B9 cells (which produce and secrete significant amounts of FGF-2), 10 x 10(6) FGF-2-A8 cells (which produce comparable amounts of FGF-2 but do not secrete it), or 10 x 10(6) control FGF-2-B8 cells (which produce only trace amounts of FGF-2) was performed after 14 days of growth, Inter-branching distances, intervascular distances, branching angles, and vessel diameters were then determined using tridimensional stereo pairs of the casted tumor vascularity. When transplanted at the same concentration, FGF-2-B9 cells grew faster in nude mice compared with FGF-2-A8 and FGF-2-B8 clones. The total amount of new vessel formation was far higher in FGF-2-B9 tumorsthan in FGF-2-B8 or FGF-2-A8 tumors. Also, vessel courses were more irregular and blind-ending vessels and evasates were more frequent in FGF-2-B9 tumors. Moreover, FGF-2-B9 tumor microvasculature was characterized by a wider average vascular diameter and by an extreme variability of the diameter of each individual vessel along its course between two ramifications, No statistical differences were observed when the distribution curves of the values of intervascular distances, interbranching distances, and branching angles of the microvessel network were compared among the different experimental groups. The distinctive features of the microvasculature of FGF-2-B9 tumors were retained, at least in part, in the smaller lesions produced by injection of a limited number of cells. The data indicate that FGF-2 production and release confer to FGF-2-B9 cells the ability to stimulate the formation of new blood vessels with distinctive architectural features. Neovascularization of FGF-2-B9 lesions parallels the faster rate of growth of the neoplastic parenchyma, This does not affect the overall architecture of the microvessel network that appears to be primed by characteristics of the HEC-1-B tumor cell line and/or by the microenvironment of the host, To our knowledge, this work represents the first attempt to define the influence of a single, defined growth factor on the tridimensional tumor vascular pattern.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 06:11:45