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Titolo:
Influence of follicle size, medium, temperature and time on the incidence of diploid bovine oocytes matured in vitro
Autore:
Ocana-Quero, JM; Pinedo-Merlin, M; Moreno-Millan, M;
Indirizzi:
Fac Vet, Dept Genet, Lab Citogenet, Cordoba 14005, Spain Fac Vet CordobaSpain 14005 t Genet, Lab Citogenet, Cordoba 14005, Spain Fac Vet, Dept Genet, Dept Patol Anim, Cordoba 14005, Spain Fac Vet Cordoba Spain 14005 Genet, Dept Patol Anim, Cordoba 14005, Spain
Titolo Testata:
THERIOGENOLOGY
fascicolo: 3, volume: 51, anno: 1999,
pagine: 667 - 672
SICI:
0093-691X(199902)51:3<667:IOFSMT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
EMBRYOS FERTILIZED INVITRO; CHROMOSOMAL-ANOMALIES; IN-VITRO; MATURATION;
Keywords:
bovine; oocytes; chromosome; in vitro maturation; serum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Ocana-Quero, JM Fac Vet, Dept Genet, Lab Citogenet, Cordoba 14005, Spain Fac Vet Cordoba Spain 14005 ogenet, Cordoba 14005, Spain
Citazione:
J.M. Ocana-Quero et al., "Influence of follicle size, medium, temperature and time on the incidence of diploid bovine oocytes matured in vitro", THERIOGENOL, 51(3), 1999, pp. 667-672

Abstract

The present work describes a cytogenetic study of in vitro-matured bovine oocytes designed to analyze the incidence of diploid oocytes induced by concentration of serum in the culture medium, follicle size, culture temperature and incubation time. In Experiment 1, immature follicular oocytes from follicles of the same size were cultured for 24 h in TCM-199 supplemented with increasing concentrations 0, 10, 20 and 50% of estrous cow serum (ECS). In Experiment 2, immature oocytes harvested from follicles of different sizes were cultured for 24 h in TCM-199 supplemented with 20% ECS at 39 degrees C in 5% CO2. In Experiment 3, immature follicular oocytes were matured inTCM-199 supplemented with 20% ECS at 2 different temperatures (37 degrees C or 39 degrees C) in 5% CO2. In Experiment 4, immature oocytes were matured over 4 different incubation times (24, 36 and 48 h) in TCM-199 supplemented with 20% ECS in 5% CO2. The highest concentration (50%) of ECS supplement in the culture medium induced the highest incidence of diploid oocytes. This incidence of diploid oocytes matured in vitro was higher in oocytes from follicles with a diameter between 11 and 15 mm. Finally, lower culture temperature (37 degrees C) and prolonged incubation time (48 h) also significantly (P<0.01) increased the percentage of diploid oocytes. (C) 1999 by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 13:51:20