Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
U2AF(35) is encoded by an essential gene clustered in an operon with RRM/cyclophilin in Caenorhabditis elegans
Autore:
Zorio, DAR; Blumenthal, T;
Indirizzi:
Univ Colorado, Hlth Sci Ctr, Dept Biochem & Mol Genet, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 ochem & Mol Genet, Denver, CO 80262 USA Indiana Univ, Dept Biol, Bloomington, IN 47405 USA Indiana Univ Bloomington IN USA 47405 ept Biol, Bloomington, IN 47405 USA
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 4, volume: 5, anno: 1999,
pagine: 487 - 494
SICI:
1355-8382(199904)5:4<487:UIEBAE>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMALL NUCLEAR RIBONUCLEOPROTEIN; COMPENSATORY BASE CHANGE; SPLICING FACTOR U2AF; C-ELEGANS; SMALL-SUBUNIT; FUNCTIONAL-ANALYSIS; AUXILIARY FACTOR; SITE SELECTION; FISSION YEAST; U1 SNRNA;
Keywords:
pre-mRNA processing; RNA interference; RNA splicing; U2 snRNA auxiliary factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Blumenthal, T Univ121,orado, Hlth Sci Ctr, Dept Biochem & Mol Genet, 4200 E 9th Ave,Box B Univ Colorado 4200 E 9th Ave,Box B121 Denver CO USA 80262 B
Citazione:
D.A.R. Zorio e T. Blumenthal, "U2AF(35) is encoded by an essential gene clustered in an operon with RRM/cyclophilin in Caenorhabditis elegans", RNA, 5(4), 1999, pp. 487-494

Abstract

In most species the 3' splice site is recognized initially by an interaction between the two-subunit splicing factor USAF with the polypyrimidine (poly(Y)) tract that results in recruitment of the U2 snRNP to the branch-point consensus just upstream. In contrast, in Caenorhabditis elegans, both thepoly(Y) tract and the branch-point consensus sequences are missing, apparently replaced by the highly conserved U(4)CAG/R 3' splice site consensus. Nevertheless C. elegans U2AF(65) is very similar to its mammalian and fly counterparts and may recognize the 3' splice site consensus. Here we report the cloning of the C. elegans U2AF(35) gene, uaf-2. We show that it lacks anidentifiable RS domain, which, in flies, has been shown to play a role in RNA binding, but it contains an extended glycine-rich stretch at its C-terminus. uaf-2 is in an operon with cyp-13, a gene that encodes a cyclophilin with an RRM domain at its N-terminus. We demonstrate by RNA interference that both U2AF genes, uaf-1 (which encodes U2AF(65)) and uaf-2, are required for viability, whereas cyp-13 is apparently not.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:22:53