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Titolo:
Trans-activation of the Tetrahymena group I intron ribozyme via a non-native RNA-RNA interaction
Autore:
Ikawa, Y; Shiraishi, H; Inoue, T;
Indirizzi:
Kyoto Univ, Fac Sci, Dept Chem, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Fac Sci, Dept Chem, Kyoto 6068502, Japan
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 7, volume: 27, anno: 1999,
pagine: 1650 - 1655
SICI:
0305-1048(19990401)27:7<1650:TOTTGI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CATALYTIC ACTIVITY; HAMMERHEAD RIBOZYME; REQUIREMENTS; ENHANCEMENT; DELETION; CLEAVAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Inoue, T Kyoto Univ, Fac Sci, Dept Chem, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Dept Chem, Kyoto 6068502, Japan
Citazione:
Y. Ikawa et al., "Trans-activation of the Tetrahymena group I intron ribozyme via a non-native RNA-RNA interaction", NUCL ACID R, 27(7), 1999, pp. 1650-1655

Abstract

The peripheral P2.1 domain of the Tetrahymena group I intron ribozyme has been shown to be non-essential for splicing. We found, however, that separately prepared P2.1 RNA efficiently accelerates the 3' splice-site-specific hydrolysis reaction of a mutant ribozyme lacking both P2.1 and its upstreamregion in trans. We report here the unusual properties of this transactivation. Compensatory mutational analysis revealed that non-native long-range base-pairings between the loop region of P2.1 RNA and L5c region of the mutant ribozyme are needed for the activation in spite of the fact that P2.1 forms base-pairings with P9.1 in the Tetrahymena ribozyme. The trans-activation depends on the non-native RNA-RNA interaction together with the higher order structure of P2.1 RNA. This activation is unique among the known trans-activations that utilize native tertiary interactions or RNA chaperons.

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Documento generato il 23/01/20 alle ore 06:17:02