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Titolo:
Diazepam binding inhibitor (DBI) gene expression in the brains of sociallyisolated and group-housed mice
Autore:
Dong, E; Matsumoto, K; Tohda, M; Kaneko, Y; Watanabe, H;
Indirizzi:
Toyamacol, & Pharmaceut Univ, Res Inst Wakan Yaku Oriental Med, Dept Pharma Toyama Med & Pharmaceut Univ Toyama Japan 9300194 ental Med, Dept Pharma
Titolo Testata:
NEUROSCIENCE RESEARCH
fascicolo: 3, volume: 33, anno: 1999,
pagine: 171 - 177
SICI:
0168-0102(199903)33:3<171:DBI(GE>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-BRAIN; STRESS; DECREASE;
Keywords:
DBI mRNA; diazepam binding inhibitor (DBI); in situ hybridization; RT-PCR; social isolation stress;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Matsumoto, K Toyamacol, & Pharmaceut Univ, Res Inst Wakan Yaku Oriental Med, Dept Pharma Toyama Med & Pharmaceut Univ 2630 Sugitani Toyama Japan 9300194
Citazione:
E. Dong et al., "Diazepam binding inhibitor (DBI) gene expression in the brains of sociallyisolated and group-housed mice", NEUROSCI RE, 33(3), 1999, pp. 171-177

Abstract

Diazepam binding inhibitor (DBI), a putative endogenous polypeptide ligandfor benzodiazepine (BZD) receptors, has been shown to act as an inverse BZD receptor agonist in the brain. We previously suggested that the social isolation stress-induced decrease in pentobarbital sleeping time in mice was partly due to an increase in the activity of endogenous substances with an inverse BZD receptor agonist-like property such as DBI. In this study, we examined whether the DBI gene expression is affected by socially isolated stress. Consistent with the previous findings, the in situ hybridization result showed very strong signals of DBI mRNA around the regions of the third ventricle, especially the lining cells, the arcuate nucleus of the hypothalamus and the cerebellum, in both socially isolated and group-housed animals. Unexpectedly, however, semi-quantitative experiments with reverse transcription-polymerase chain reaction technique revealed that socially isolated mice had significantly less expression of DBI mRNA in the hypothalamus than group-housed animals, and no difference in the expression in the other brain areas was observed between two animal groups. We discuss the relationshipbetween the decrease of DBI mRNA expression in the hypothalamus and the decrease of GABA(A) receptor function following long-term social isolation inmice. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 11:56:39