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Titolo:
Optical imaging of the spatiotemporal patterning of neural responses in the embryonic chick superior cervical ganglion
Autore:
Momose-Sato, Y; Komuro, H; Hirota, A; Sakai, T; Sato, K; Kamino, K;
Indirizzi:
Tokyoaned & Dent Univ, Sch Med, Dept Physiol, Bunkyo Ku, Tokyo 1138519, Jap Tokyo Med & Dent Univ Tokyo Japan 1138519 Bunkyo Ku, Tokyo 1138519, Jap
Titolo Testata:
NEUROSCIENCE
fascicolo: 3, volume: 90, anno: 1999,
pagine: 1069 - 1083
SICI:
0306-4522(1999)90:3<1069:OIOTSP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
VOLTAGE-SENSITIVE DYES; BRAIN-STEM; ELECTRICAL-ACTIVITY; POSTSYNAPTIC POTENTIALS; SYNAPTIC TRANSMISSION; SYMPATHETIC NEURONS; VAGAL-STIMULATION; COMPONENTS; SYSTEM;
Keywords:
superior cervical ganglion; development; synaptogenesis; EPSP; optical imaging; voltage-sensitive dye;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Momose-Sato, Y Tokyoaned & Dent Univ, Sch Med, Dept Physiol, Bunkyo Ku, Tokyo 1138519, Jap Tokyo Med & Dent Univ Tokyo Japan 1138519 yo 1138519, Jap
Citazione:
Y. Momose-Sato et al., "Optical imaging of the spatiotemporal patterning of neural responses in the embryonic chick superior cervical ganglion", NEUROSCIENC, 90(3), 1999, pp. 1069-1083

Abstract

Multiple-site optical recording of transmembrane potential changes with a voltage-sensitive dye was used to reveal the functional expression and developmental changes of the postsynaptic potentials in the early embryonic chick superior cervical ganglion. The ganglia were isolated from five- to 12-day-old chick embryos with preganglionic nerve fibres (vertebral and/or cervical carotic nerves) attached. The preparations were stained with a voltage-sensitive merocyanine-rhodanine dye (NK2761). Voltage-related optical (absorbance) changes were recorded simultaneously from 127 contiguous loci in the preparation, using a 12 x 12-element photodiode array. Optical changes having two components were evoked by preganglionic nerve stimulation. One component was the fast spike-like signal and another the delayed slow signal. The amplitude of the slow signal was decreased by repetitive stimulation, reduced by low external calcium ion concentrations and eliminated in the presence of manganese or cadmium ions. The slow signals were also eliminated in the presence of D-tubocurarine. Accordingly, we concluded that the slow signal corresponds to cholinergic excitatory postsynaptic potentials. In the five- and six-day-old superior cervical ganglia, only the fast optical signals (referred to as the action potentials) were recorded. Slow optical signals (referred to as the excitatory postsynaptic potentials) were detectedfrom preparations older than seven days. The amplitude of the slow opticalsignal gradually increased, together with an expansion of the response area, as the developmental stage proceeded from seven to 10 days. To compare the distribution patterns of the neural responses evoked by stimuli applied to the cervical carotic and vertebral nerves, we have mapped and imaged thespatial patterning of the synaptic responses. In the maps, the positions of the peak size regions of the slow signals were assessed, and we found that there were differences in the location of these areas for the cervical carotic vs vertebral nerves. From these experimental results, we conclude that synaptic function withinthe chick superior cervical ganglion is initiated at the seven-day-old embryonic stage, and reaches a maximum level at 10 days. Synaptic transmissionat these stages is mediated solely by nicotinic acetylcholine receptors. The spatial mapping of the synaptic responses reveals that the neural populations related synaptically to the cervical carotic and vertebral nerves arelocated separately within the ganglion, even at an early developmental stage. (C) 1999 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 14:17:47