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Titolo:
Plakophilin, armadillo repeats, and nuclear localization
Autore:
Klymkowsky, MW;
Indirizzi:
Univ Colorado, Boulder, CO 80309 USA Univ Colorado Boulder CO USA 80309Univ Colorado, Boulder, CO 80309 USA
Titolo Testata:
MICROSCOPY RESEARCH AND TECHNIQUE
fascicolo: 1, volume: 45, anno: 1999,
pagine: 43 - 54
SICI:
1059-910X(19990401)45:1<43:PARANL>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEGMENT POLARITY GENE; TRANSCRIPTION FACTOR LEF-1; GREEN FLUORESCENT PROTEIN; BETA-CATENIN; DESMOSOMAL PLAQUE; XENOPUS EMBRYOS; AXIS FORMATION; FAMILY MEMBER; FROG XENOPUS; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Klymkowsky, MW Univ Colorado, Boulder, CO 80309 USA Univ Colorado BoulderCO USA 80309 , Boulder, CO 80309 USA
Citazione:
M.W. Klymkowsky, "Plakophilin, armadillo repeats, and nuclear localization", MICROSC RES, 45(1), 1999, pp. 43-54

Abstract

Plakophilins are armadillo-repeat containing proteins, identified through their localization to desmosomes. Expressed in a wide range of tissues, plakophilins are largely nuclear in most cell types [Schmidt et al. (1997) Cell Tissue Res 290:481; Mertens et al. (1996) J Cell Biol 135:1009]. Using Xenopus embryos and cultured A6 cells, together with myc- and green fluorescent protein (GFP)-tags, we found that both the N-terminal, non-armadillo repeat "head" and the C-terminal armadillo repeat-containing regions can enternuclei. The "arm" repeat domain is predominantly cytoplasmic and concentrated at the cell cortex, whereas the head and full-length polypeptides are concentrated in the nucleus. The head domain can also be seen to decorate and disrupt keratin filament network organization in some cells. In the course of these studies, we found that the distribution of the myc-epitope and green fluorescence differed in fixed cells, e.g., while the green fluorescence of a myc- and GFP-tagged head domain polypeptide was usually exclusivelynuclear, a substantial fraction of the myc-immunoreactivity was cytoplasmic. Treating cells with the translation inhibitor cycloheximide reduces the cytoplasmic myc-signal, suggesting that it represented nascent polypeptidesawaiting folding and nuclear import. Based on these types of experiments, GFP can be seen as a marker of the distribution of the mature form of the tagged polypeptide. (C) 1999 Wiley-Liss, Inc.

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Documento generato il 07/07/20 alle ore 19:52:47