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Titolo:
Characterisation of feruloyl esterase activity in barley
Autore:
Sancho, AI; Faulds, CB; Bartolome, B; Williamson, G;
Indirizzi:
AFRC, Inst Food Res, Dept Biochem, Norwich NR4 7UA, Norfolk, England AFRCNorwich Norfolk England NR4 7UA m, Norwich NR4 7UA, Norfolk, England CSIC, Inst Fermentac Ind, E-28006 Madrid, Spain CSIC Madrid Spain E-28006 SIC, Inst Fermentac Ind, E-28006 Madrid, Spain
Titolo Testata:
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
fascicolo: 3, volume: 79, anno: 1999,
pagine: 447 - 449
SICI:
0022-5142(19990301)79:3<447:COFEAI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-WALL POLYSACCHARIDES; PHENOLIC COMPONENTS; PARA-COUMAROYL; ACID; RELEASE; LINKAGE;
Keywords:
barley; feruloyl esterase; ferulic acid; germination;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Faulds, CB AFRC,,Inst Food Res, Dept Biochem, Norwich Res Pk, Norwich NR4 7UA, Norfolk AFRC Norwich Res Pk Norwich Norfolk England NR4 7UA UA, Norfolk
Citazione:
A.I. Sancho et al., "Characterisation of feruloyl esterase activity in barley", J SCI FOOD, 79(3), 1999, pp. 447-449

Abstract

Feruloyl esterase activity has been detected in barley grain and germinated barley. No differences have been found between the values of activity against methyl ferulate in barley crude extracts (1.05 +/- 0.10 mU mg(-1) protein) with and without sterilisation (P > 0.05), indicating that the activity is native to the barley grain rather than due to microbial contamination. The highest value of esterase activity was detected after 2 day growth, although an overall downward trend was observed over a 6 day germination period at 25 degrees C. The K-m and V-max of the crude barley esterase/s on methyl ferulate were 0.34 +/- 0.05 mM and 0.76 +/- 0.03 mU mg(-1) protein, respectively. The crude extracts were also active upon the methyl esters of sinapic, p-coumaric and caffeic acids, and upon a feruloylated arabino-oligosaccharide (Ara(2)F), but not upon the methyl ester of vanillic acid. (C) 1999 Society of Chemical Industry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 06:00:35