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Titolo:
Release of ferulic acid dehydrodimers from plant cell walls by feruloyl esterases
Autore:
Kroon, PA; Garcia-Conesa, MT; Fillingham, IJ; Hazlewood, GP; Williamson, G;
Indirizzi:
AFRC, Inst Food Res, Dept Biochem, Norwich NR4 7UA, Norfolk, England AFRCNorwich Norfolk England NR4 7UA m, Norwich NR4 7UA, Norfolk, England Babraham Inst, Lab Mol Enzymol, Cambridge CB2 4AT, England Babraham Inst Cambridge England CB2 4AT ymol, Cambridge CB2 4AT, England
Titolo Testata:
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
fascicolo: 3, volume: 79, anno: 1999,
pagine: 428 - 434
SICI:
0022-5142(19990301)79:3<428:ROFADF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUGAR-BEET PULP; FLUORESCENS SUBSP CELLULOSA; ASPERGILLUS-NIGER; DIFERULIC ACID; WHEAT BRAN; DEHYDRODIFERULIC ACIDS; MAIZE COLEOPTILES; GROWTH; OLIGOSACCHARIDES; IDENTIFICATION;
Keywords:
ferulic acid; diferulic acids; dehydrodiferulic acids; feruloyl esterases; wheat bran; sugar beet; plant cell walls; phenolic cross-links;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Kroon, PA Instandod Res, Dept Biochem, Norwich Res Pk, Norwich NE4 7UA, Norfolk, Engl Inst Food Res Norwich Res Pk Norwich Norfolk England NE4 7UA ngl
Citazione:
P.A. Kroon et al., "Release of ferulic acid dehydrodimers from plant cell walls by feruloyl esterases", J SCI FOOD, 79(3), 1999, pp. 428-434

Abstract

Dehydrodimers of hydroxycinnamic acids, such as ferulic and p-coumaric acids, are important structural components which serve to cross-link polymers in plant cell walls. Dehydrodiferulate oligosaccharide diesters were solubilised from wheat bran or sugar beet pulp by treatment with Driselase and release of dehydrodiferulic free acids from this substrate by purified feruloyl esterases was analysed by HPLC. We detected 5-5diFA, 8-O-4diFA and 8-5BendiFA in saponified extracts from wheat bran and sugar beet pulp. Driselase-treatment solubilised 21 and 97% of the saponifiable dehydrodimers from wheat bran and sugar beet pulp, respectively, but only as dehydrodiferulate esters, not as dehydrodiferulic acids. At low concentrations, feruloyl esterase A (FAEA) from Aspergillus niger and an esterase (Xy1D) from Pseudomonasfluorescens released 93% and 36% of the saponifiable 5-5diFA from solubilised wheat bran, respectively, but only 12 and 15% from solubilised sugar beet pulp. At higher concentrations, only FAEA also released 8-O-4diFA (65%) from solubilised wheat bran, but not sugar beet pulp. We could not detect release of any dehydrodiferulic acids from solubilised wheat bran or sugar beet pulp using either a cinnamoyl ester hydrolase from Piromyces equi (CEH)or from A niger (CinnAE). Our results demonstrate that FAEA and Xy1D hydrolyse dehydrodiferulate diesters to release the free acids, which means theyhave the potential to break the putative cross-links present in graminaceous monocot and dicot cell walls, while two other feruloyl esterases (CinnAEand CEH) did not release dehydrodiferulic acids from plant cell walls. (C)1999 Society of Chemical Industry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 12:03:56