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Titolo:
Simultaneous SPECT studies of pre- and postsynaptic dopamine binding sitesin baboons
Autore:
Dresel, SHJ; Kung, MP; Huang, XF; Plossl, K; Hou, C; Meegalla, SK; Patselas, G; Mu, M; Saffer, JR; Kung, HF;
Indirizzi:
Univ Penn, Dept Radiol, Philadelphia, PA 19104 USA Univ Penn PhiladelphiaPA USA 19104 pt Radiol, Philadelphia, PA 19104 USA
Titolo Testata:
JOURNAL OF NUCLEAR MEDICINE
fascicolo: 4, volume: 40, anno: 1999,
pagine: 660 - 666
SICI:
0161-5505(199904)40:4<660:SSSOPA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOTON-EMISSION TOMOGRAPHY; MONITORING COGNITIVE ACTIVATION; TRANSPORTER IMAGING AGENT; PARKINSONS-DISEASE; ATYPICAL NEUROLEPTICS; RECEPTOR OCCUPANCY; WILSONS-DISEASE; TC-99M COMPLEX; I-123 IBZM; BRAIN;
Keywords:
dopamine transporters; dopamine D-2/D-3 receptors; SPECT imaging; dual-isotope studies;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Dresel, SHJ Univ Penn, Dept Radiol, 3700 Market St,Room 305, Philadelphia,PA 19104 USA Univ Penn 3700 Market St,Room 305 Philadelphia PA USA 19104 SA
Citazione:
S.H.J. Dresel et al., "Simultaneous SPECT studies of pre- and postsynaptic dopamine binding sitesin baboons", J NUCL MED, 40(4), 1999, pp. 660-666

Abstract

The central nervous system dopamine transporters (DATs) and dopamine D-2/D-3, receptors are implicated in a variety of neurological disorders. Both sites are also targets for drug treatment. With the successful development of [(TC)-T-99m]TRODAT-1 single-isotope imaging studies using this ligand forDAT imaging can be complemented by additional use of I-123-labeled D-2/D-3, receptor ligand co-injected to assess both pre- and postsynaptic sites ofthe dopaminergic system simultaneously. Methods: Twelve SPECT scans of thebrain were obtained in two baboons after intravenous administration of 740MBq (20 mCi) [Tc-99m]TRODAT-1 (technetium, [2-[[2-[[[3-(4-chlorophenyl)-8-methyl-8-azabicyclo[3,2,1]oct-2-yl]methyl](2-mercaptoethyl) amino]ethyl]amino]ethanethiolato (3-)]- oxo-[1R-(exo-exo)]) and 185 MBq (5 mCi) [I-123]iodobenzamide or [I-123]iodobenzofuran. SPECT data were acquired by a triple-head gamma camera equipped with ultra-high-resolution fanbeam collimators (scan duration = 210 min). Two sets of SPECT data were obtained using energy windows of 15% centered on 140 keV for Tc-99m and 10% asymmetric with a lower bound at 159 keV for I-123. After coregistration with MRI, region-of-interest analysis was performed using predefined templates from coregistered MRI. In blocking studies, baboons were pretreated with N-methyl-3 beta-carbomethoxy-3 beta-(4-fluorophenyl)tropane (CFT, 14 mg) or raclopride (14 mg) to block DAT or D-2/D-3, binding site, respectively. Results: Image quality of dual-isotope studies was similar to that obtained from single-isotope studies. When one site was blocked with CFT or raclopride, the binding of therespective ligand to the other site was not affected. Conclusion: This is the first example that clearly demonstrates the feasibility of simultaneousimaging of both pre- and postsynaptic sites of the dopaminergic system in baboons with dual-isotope SPECT studies. With or without corrections for cross-contamination of I-123 into the Tc-99m window, striatum-to-cerebellum ratios (target-to-nontarget) of dual-isotope experiments did not differ significantly from single-isotope experiments. This method may be a valuable and cost-effective tool for gaining comprehensive information about the dopaminergic system in one SPECT imaging session.

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Documento generato il 24/01/20 alle ore 12:20:06