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Titolo:
SNARE membrane trafficking dynamics in vivo
Autore:
Chao, DS; Hay, JC; Winnick, S; Prekeris, R; Klumperman, J; Scheller, RH;
Indirizzi:
Stanford,Univ, Sch Med, Howard Hughes Med Inst, Dept Mol & Cellular Physiol Stanford Univ Stanford CA USA 94305 Med Inst, Dept Mol & Cellular Physiol Univ Utrecht, Sch Med, Inst Biomembranes, NL-3584 CX Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3584 CX 3584 CX Utrecht, Netherlands
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 5, volume: 144, anno: 1999,
pagine: 869 - 881
SICI:
0021-9525(19990308)144:5<869:SMTDIV>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYNAPTIC VESICLE DOCKING; GOLGI-APPARATUS; LIVING CELLS; PROTEIN; FUSION; TRANSPORT; COMPLEX; YEAST; ER; GLYCOPROTEIN;
Keywords:
vesicle trafficking; fluorescent proteins; SNAREs; membrane proteins; endoplasmic reticulum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Scheller, RH Stanford,Univ, Sch Med, Howard Hughes Med Inst, Dept Mol & Cellular Physiol Stanford Univ Stanford CA USA 94305 t Mol & Cellular Physiol
Citazione:
D.S. Chao et al., "SNARE membrane trafficking dynamics in vivo", J CELL BIOL, 144(5), 1999, pp. 869-881

Abstract

The ER/Golgi soluble NSF attachment protein receptor (SNARE) membrin, rsec22b, and rbet1 are enriched in similar to 1-mu m cytoplasmic structures that lie very close to the ER, These appear to be ER exit sites since secretory cargo concentrates in and exits from these structures. rsec22b and rbet1 fused to fluorescent proteins are enriched at similar to 1-mu m ER exit sites that remained more or less stationary, but periodically emitted streaks of fluorescence that traveled generally in the direction of the Golgi complex. These exit sites were reused and subsequent tubules or streams of vesicles followed similar trajectories. Fluorescent membrin-enriched similar to 1-mu m peripheral structures were more mobile and appeared to translocate through the cytoplasm back and forth, between the periphery and the Golgi area, These mobile structures could serve to collect secretory cargo by fusing with ER-derived vesicles and ferrying the cargo to the Golgi. The post-Golgi SNAREs, syntaxin 6 and syntaxin 33, when fused to fluorescent proteins each displayed characteristic patterns of movement. However, syntaxin 13 was the only SNARE whose life cycle appeared to involve interactions with theplasma membrane, These studies reveal the in vivo spatiotemporal dynamics of SNARE proteins and provide new insight into their roles in membrane trafficking.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:55:12