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Titolo:
Genetic localization and molecular characterization of two key genes (mitAB) required for biosynthesis of the antitumor antibiotic mitomycin C
Autore:
Mao, YQ; Varoglu, M; Sherman, DH;
Indirizzi:
Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 robiol, Minneapolis, MN 55455 USA Univ Minnesota, Biol Proc Technol Inst, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 l Inst, Minneapolis, MN 55455 USA
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 7, volume: 181, anno: 1999,
pagine: 2199 - 2208
SICI:
0021-9193(199904)181:7<2199:GLAMCO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
3-AMINO-5-HYDROXYBENZOIC ACID; ESCHERICHIA-COLI; ANSAMYCIN ANTIBIOTICS; STREPTOMYCES-LAVENDULAE; CLONING VECTORS; MC(7)N UNITS; RESISTANCE; PRECURSOR; CLUSTER; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Sherman, DH UniveMinnesota, Dept Microbiol, Box 196 UFHC,1460 Mayo Mem Bldg,420 Delawar Univ Minnesota Box 196 UFHC,1460 Mayo Mem Bldg,420 Delaware St S Minneapolis MN USA 55455
Citazione:
Y.Q. Mao et al., "Genetic localization and molecular characterization of two key genes (mitAB) required for biosynthesis of the antitumor antibiotic mitomycin C", J BACT, 181(7), 1999, pp. 2199-2208

Abstract

Mitomycin C (MC) is an antitumor antibiotic derived biosynthetically from 3-amino-5-hydroxybenzoic acid (AHBA), D glucosamine, and carbamoyl phosphate. A gene (mitA) involved in synthesis of AHBA has been identified and found to be linked to the MC resistance locus, mrd, in Streptomyces lavendulae. Nucleotide sequence analysis showed that mitA encodes a 388-amino-acid protein that has 71% identity (80% similarity) with the rifamycin AHBA synthase from Amycolatopsis mediterranei, as well as,vith two additional AHBA synthases from related ansamycin antibiotic-producing microorganisms. Gene disruption and site-directed mutagenesis of the S. lavendulae chromosomal copy of mitA completely blocked the production of MC. The function of mitA was confirmed by complementation of an S. lavendulae strain containing a K191A mutation in MitA with AHBA. A second gene (mitB) encoding a 272-amino-acid protein (related to a group of glycosyltransferases) was identified immediately downstream of mitA that upon disruption resulted in abrogation of MC synthesis. This work has localized a cluster of key genes that mediate assembly of the unique mitosane class of natural products.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 12:54:40