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Titolo:
Bacteroides fragilis toxin 2 damages human colonic mucosa in vitro
Autore:
Riegler, M; Lotz, M; Sears, C; Pothoulakis, C; Castagliuolo, I; Wang, CC; Sedivy, R; Sogukoglu, T; Cosentini, E; Bischof, G; Feil, W; Teleky, B; Hamilton, G; LaMont, JT; Wenzl, E;
Indirizzi:
Harvardston,, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Bo Harvard Univ Boston MA USA 02215 Deaconess Med Ctr, Div Gastroenterol, Bo Univ Vienna, Univ Clin Surg, Vienna, Austria Univ Vienna Vienna AustriaUniv Vienna, Univ Clin Surg, Vienna, Austria Univ Vienna, Inst Clin Pathol, Vienna, Austria Univ Vienna Vienna Austria iv Vienna, Inst Clin Pathol, Vienna, Austria Danube Hosp, Dept Surg, Vienna, Austria Danube Hosp Vienna AustriaDanube Hosp, Dept Surg, Vienna, Austria Johns Hopkins Univ, Div Infect Dis, Baltimore, MD USA Johns Hopkins Univ Baltimore MD USA v, Div Infect Dis, Baltimore, MD USA Johns Hopkins Univ, Div Gastroenterol, Baltimore, MD USA Johns Hopkins Univ Baltimore MD USA Div Gastroenterol, Baltimore, MD USA
Titolo Testata:
GUT
fascicolo: 4, volume: 44, anno: 1999,
pagine: 504 - 510
SICI:
0017-5749(199904)44:4<504:BFT2DH>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITHELIAL-CELLS; IN-VITRO; DIARRHEAL DISEASE; METALLOPROTEASE TOXIN; RHO-PROTEINS; ENTEROTOXIN; ACTIN; ENTEROVIRULENCE; ASSOCIATION; HT29/C1;
Keywords:
B fragilis toxin; toxin mediated colonocyte damage; actin filaments; transepithelial resistance; morphometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Pothoulakis, C Harvard0Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, 33 Harvard Univ 330 Brookline Ave Boston MA USA 02215 erol, 33
Citazione:
M. Riegler et al., "Bacteroides fragilis toxin 2 damages human colonic mucosa in vitro", GUT, 44(4), 1999, pp. 504-510

Abstract

Background-Strains of Bacteroides fragilis producing a 20 kDa protein toxin (B fragilis toxin (BFT) or fragilysin) are associated with diarrhoea in animals and humans. Although in vitro results indicate that EFT damages intestinal epithelial cells in culture, the effects of BFT on native human colon are not known. Aims-To examine the electrophysiological and morphological effects of purified BFT-2 on human colonic mucosa in vitro. Methods-For resistance (EP) measurements, colonic mucosa mounted in Ussingchambers was exposed to luminal or serosal BFT-2 (1.25-10 nM) and after four hours morphological damage was measured on haematoxylin and eosin stained sections using morphometry. F actin distribution was assessed using confocal microscopy. Results-Serosal BFT-2 for four hours was four-, two;, seven-, and threefold more potent than luminal BFT-2 in decreasing resistance, increasing epithelial H-3-mannitol permeability, and damaging crypt and surface colonocytes, respectively. (p<0.05). Confocal: microscopy showed reduced colonocyte F actin staining intensity after exposure to BFT-2. Conclusions-BFT-2 increases human colonic permeability and damages human colonic epithelial cells in vitro. These effects may be important in the development of diarrhoea and intestinal inflammation caused by B fragilis in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:35:20