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Titolo:
Involvement of Rab4 in regulated exocytosis of rat pancreatic acini
Autore:
Ohnishi, H; Mine, T; Shibata, H; Ueda, N; Tsuchida, T; Fujita, T;
Indirizzi:
Univ Tokyo, Sch Med, Dept Internal Med 4, Bunkyo Ku, Tokyo 1128688, Japan Univ Tokyo Tokyo Japan 1128688 al Med 4, Bunkyo Ku, Tokyo 1128688, Japan Gunma1,niv, Inst Mol & Cellular Regulat, Dept Cell Biol, Maebashi, Gumma 37 Gunma Univ Maebashi Gumma Japan 371 t, Dept Cell Biol, Maebashi, Gumma 37
Titolo Testata:
GASTROENTEROLOGY
fascicolo: 4, volume: 116, anno: 1999,
pagine: 943 - 952
SICI:
0016-5085(199904)116:4<943:IORIRE>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTERIOR-PITUITARY-CELLS; BINDING PROTEIN RAB4; GLUCOSE-TRANSPORT; ZYMOGEN GRANULES; PHOSPHATIDYLINOSITOL 3-KINASE; CA2+-DEPENDENT EXOCYTOSIS; SYNAPTIC VESICLES; TERMINAL DOMAIN; PLASMA-MEMBRANE; KINASE-C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Ohnishi, H UnivoTokyo, Sch Med, Dept Internal Med 4, Bunkyo Ku, 3-28-6 Mejirodai, Toky Univ Tokyo 3-28-6 Mejirodai Tokyo Japan 1128688 ejirodai, Toky
Citazione:
H. Ohnishi et al., "Involvement of Rab4 in regulated exocytosis of rat pancreatic acini", GASTROENTY, 116(4), 1999, pp. 943-952

Abstract

Background & Aims: Rab4, a Ras-related small guanosine triphosphate (GTP)-binding protein, has been suggested to participate in exocytosis. The function of Rab4 in regulated exocytosis of pancreatic acini was examined in this study. Methods: Subcellular localization of Rab4 was determined by Western blotting and immunohistochemistry. The Rab4 function in regulated exocytosis was examined by introducing Rab4 hypervariable carboxy-terminal domain peptide (Rab4 peptide) and anti-Rab4 antibody into streptolysin O-permeabilized acini, The regulation of Rab4 by cholecystokinin (CCK) and 12-O-tetradecanoyl-phorbol 13-acetate (TPA) was investigated by examining their effects on [P-32]GTP binding rate into the Rab4 immunoprecipitates. The participation of protein kinase C in the Rab4 regulation by CCK was confirmed by calphostin C pretreatment of acini. Results: Rab4 was localized on zymogen granule membranes. Both Rab4 peptide and anti-Rab4 antibody enhanced calcium-stimulated amylase release from streptolysin O-permeabilized acini, suggesting the inhibitory role of Rab4 in exocytosis. CCK and TPA increased GTP binding to Rab4. Calphostin C attenuated the stimulatory effect of CCK on GTP binding to Rab4. Conclusions: Rab4 negatively modulates regulated exocytosis of pancreatic acini and is controlled by CCK through a protein kinase C pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 23:12:36