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Titolo:
Bacterial proteins carrying twin-R signal peptides are specifically targeted by the Delta pH-dependent transport machinery of the thylakoid membrane system
Autore:
Halbig, D; Hou, B; Freudl, R; Sprenger, GA; Klosgen, RB;
Indirizzi:
Univ Halle Wittenberg, Inst Pflanzen & Zellphysiol, D-06108 Halle, GermanyUniv Halle Wittenberg Halle Germany D-06108 siol, D-06108 Halle, Germany KFAanylich GmbH, Forschungszentrum, Inst Biotechnol 1, D-52425 Julich, Germ KFA Julich GmbH Julich Germany D-52425 iotechnol 1, D-52425 Julich, Germ
Titolo Testata:
FEBS LETTERS
fascicolo: 1, volume: 447, anno: 1999,
pagine: 95 - 98
SICI:
0014-5793(19990319)447:1<95:BPCTSP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE-FRUCTOSE OXIDOREDUCTASE; ZYMOMONAS-MOBILIS; PATHWAY SPECIFICITY; ESCHERICHIA-COLI; PROTON GRADIENT; TRANSLOCATION; AZIDE; GENE; CHLOROPLASTS; EXPRESSION;
Keywords:
glucose-fructose oxidoreductase; protein transport; bacterial export; thylakoid membrane; Delta pH-dependent pathway; twin-arginine motif;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Klosgen, RB Univle,lle Wittenberg, Inst Pflanzen & Zellphysiol, Kirchtor 1, D-06108 Hal Univ Halle Wittenberg Kirchtor 1 Halle Germany D-06108 08 Hal
Citazione:
D. Halbig et al., "Bacterial proteins carrying twin-R signal peptides are specifically targeted by the Delta pH-dependent transport machinery of the thylakoid membrane system", FEBS LETTER, 447(1), 1999, pp. 95-98

Abstract

Glucose-fructose oxidoreductase (GFOR), a periplasmic protein of Zymomonasmobilis, is synthesized as a precursor polypeptide with a twin-R signal peptide for Sec-independent protein export in bacteria. In higher plant chloroplasts, twin-R signal peptides are specific targeting signals for the Sec-independent Delta pH pathway of the thylakoid membrane system. In agreementwith the assumed common phylogenetic origin of the two protein transport mechanisms, GFOR can be efficiently translocated by the Delta pH-dependent pathway when analyzed with isolated thylakoid membranes. Transport is sensitive to the ionophore nigericin and competes with specific substrates for the Delta pH-dependent transport route. In contrast, neither sodium azide norenzymatic destruction of the nucleoside triphosphates in the assays affects thylakoid transport of GFOR indicating that the Sec apparatus is not involved in this process. Mutagenesis of the twin-R motif in the GFOR signal peptide prevents membrane translocation of the protein emphasizing the importance of these residues for the transport process. (C) 1999 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 20:33:31