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Titolo:
Binding and two-dimensional crystallization of streptavidin at the air/water interface via engineered Cu-IDA chelator lipids
Autore:
Maloney, KM; Schief, WR; Pack, DW; Frey, W; Arnold, FH; Vogel, V;
Indirizzi:
Univ Washington, Dept Bioengn, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 Dept Bioengn, Seattle, WA 98195 USA CALTECH, Dept Chem & Chem Engn, Pasadena, CA 91125 USA CALTECH Pasadena CA USA 91125 pt Chem & Chem Engn, Pasadena, CA 91125 USA
Titolo Testata:
COORDINATION CHEMISTRY REVIEWS
, volume: 183, anno: 1999,
pagine: 3 - 18
SICI:
0010-8545(199903)183:<3:BATCOS>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
AIR-WATER-INTERFACE; 2-DIMENSIONAL PROTEIN CRYSTALLIZATION; METAL-AFFINITY CHROMATOGRAPHY; BREWSTER-ANGLE MICROSCOPY; CONTAINING CYTOCHROME-C; DIRECT VISUALIZATION; SURFACE HISTIDINES; CORE STREPTAVIDIN; PHASE-TRANSITIONS; MONOLAYERS;
Keywords:
air/water interface; Brewster angle microscopy; Cu(II); fluorescence microscopy; iminodiacetate; metal-chelating lipids; monolayers;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Vogel, V Univ Washington, Dept Bioengn, Box 352125, Seattle, WA 98195 USA Univ Washington Box 352125 Seattle WA USA 98195 tle, WA 98195 USA
Citazione:
K.M. Maloney et al., "Binding and two-dimensional crystallization of streptavidin at the air/water interface via engineered Cu-IDA chelator lipids", COORD CH RE, 183, 1999, pp. 3-18

Abstract

Metal-chelating iminodiacetate (IDA) lipid monolayers at the air/water interface have been engineered to promote the 2D crystallization of proteins which display solvent-accessible histidines. The physical properties of IDA lipid monolayers have been specifically tailored to allow the binding and 2D assembly of added proteins. Fluorescently labeled IDA lipid monolayers allow us to monitor the formation of the protein crystals optically. Here we review our efforts in the interfacial crystallization of streptavidin as monitored using fluorescence, Brewster angle and transmission electron microscopy and discuss future challenges in this area. (c) 1999 Elsevier Science S.A. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:54:18