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Titolo:
Carob is not allergenic in peanut-allergic subjects
Autore:
Fiocchi, A; Restani, P; Travaini, M; Decet, E; Gaiaschi, A; Bernardo, L; Riva, E;
Indirizzi:
Univ Milan, Dept Paediat, San Paolo Biomed Inst, I-20142 Milan, Italy UnivMilan Milan Italy I-20142 n Paolo Biomed Inst, I-20142 Milan, Italy Univ Milan, Inst Pharmacol Sci, Toxicol Lab, Milan, Italy Univ Milan Milan Italy n, Inst Pharmacol Sci, Toxicol Lab, Milan, Italy
Titolo Testata:
CLINICAL AND EXPERIMENTAL ALLERGY
fascicolo: 3, volume: 29, anno: 1999,
pagine: 402 - 406
SICI:
0954-7894(199903)29:3<402:CINAIP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEGUME BOTANICAL FAMILY; ATOPIC-DERMATITIS; FOOD HYPERSENSITIVITY; CROSS-ALLERGENICITY; CHILDREN; IDENTIFICATION; CHALLENGES; EXTRACTS;
Keywords:
carob allergy; cross-reactivity; double-blind placebo-controlled food challenge; immunological tolerance; peanut allergy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Fiocchi, A Univilan,n, Dept Paediat, San Paolo Biomed Inst, Via Di Rudini 8, I-20142 M Univ Milan Via Di Rudini 8 Milan Italy I-20142 ni 8, I-20142 M
Citazione:
A. Fiocchi et al., "Carob is not allergenic in peanut-allergic subjects", CLIN EXP AL, 29(3), 1999, pp. 402-406

Abstract

Background The antigenic potential of proteins from the carob bean, a member of the legume family used as a food additive, have not so far been investigated and legumes share antigenic proteins with peanut, a potent trigger of anaphylaxis. Objective To assess the carob protein determinants of sensitization in peanut-allergic children. Methods In a prospective, double-blind, placebo-controlled study I? patients (median age 9.5 years) with a history of hyperreactivity to peanut (anaphylaxis) were assessed. Skin prick tests with a commercial peanut allergen,raw carob pulp, raw and cooked carob cotyledon formula were used to confirm the history. RAST for peanuts and cooked carob were used to evaluate sensitization to these proteins. Carob-specific IgE were identified by immunoblotting analyses. Allergic reactivity was evaluated during double-blind placebo-controlled food challenges (DBPCFC: 5.5 g carob extract and cooked carob cotyledon formula). Results Peanut allergen-induced skin prick test positivity in all children(confirmed during double-blinded challenge in 6/12 patients), carob pulp in 3/12 patients, raw carob bean in 6/ 12, and cooked carob cotyledon formula in none. RAST were positive for peanut in all cases but negative for carob beans in 9/12 cases. Immunoblot analyses found peanut-specific IgE in allcases and raw carob bean-specific IgE in eight cases. Carob allergens wereidentified in the 17.5, 48, and 66 kDa MW bands. The least allergenic density was found for cooked carob proteins. There was no clinical reactivity with either raw or cooked carob during DBPCFC. Conclusions These data suggest that carob-specific sensitization, apparentboth in vitro and in SPTs, can be concordant with peanut allergy and that cooked carob can be ingested by children who are allergic to peanuts. That heat-processing deactivates carob protein allergenicity has dietary implications for polyallergic children.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 08:07:59