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Titolo:
Increased adhesion of the promyelocytic leukaemia cell line, NB4, to fibronectin and thrombospondin upon all-trans-retinoic acid treatment
Autore:
Touhami, M; Bourge, JF; Legrand, C;
Indirizzi:
Hop St Louis, INSERM, U353, F-75475 Paris 10, France Hop St Louis Paris France 10 uis, INSERM, U353, F-75475 Paris 10, France Hop St Louis, INSERM, U462, Inst Hematol, F-75475 Paris, France Hop St Louis Paris France F-75475 2, Inst Hematol, F-75475 Paris, France
Titolo Testata:
BRITISH JOURNAL OF HAEMATOLOGY
fascicolo: 4, volume: 104, anno: 1999,
pagine: 706 - 714
SICI:
0007-1048(199903)104:4<706:IAOTPL>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC PROGENITOR CELLS; BONE-MARROW STROMA; INTEGRIN-ASSOCIATED PROTEIN; HEPARIN-BINDING DOMAIN; PLATELET THROMBOSPONDIN; EXTRACELLULAR-MATRIX; SIGNAL-TRANSDUCTION; LEUKEMIA; DIFFERENTIATION; MODULATION;
Keywords:
fibronectin; thrombospondin; adhesion; NB4 cells; retinoic acid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Legrand, C HopeSt Louis, INSERM, U353, 1 Ave Claude Vellefaux, F-75475 Paris 10, Franc Hop St Louis 1 Ave Claude Vellefaux Paris France 10 10, Franc
Citazione:
M. Touhami et al., "Increased adhesion of the promyelocytic leukaemia cell line, NB4, to fibronectin and thrombospondin upon all-trans-retinoic acid treatment", BR J HAEM, 104(4), 1999, pp. 706-714

Abstract

We have evaluated the effects of all-trans-retinoic acid (RA) on the adhesion of the human promyelocytic tic cell Line NB4 to various components of the extracellular matrix. NB4 cells, radiolabelled with (111)Indium, showed a 2-3-fold increase(P < 0.001) in adhesion to fibronectin and thrombospondin upon RA (3x10(-7) M) treatment, whereas adhesion to collagen I. laminin and vitronectin was not modified, The increase in cell adhesion, observed asearly as day 1, preceded cell differentiation and was concomitant with tyrosine phosphorylation events. Using now cytometry, we analysed the expression of major receptors for fibronectin (alpha 4 beta 1 and alpha 5 beta 1) and for thrombospondin (alpha nu beta 3, alpha IIb beta 3. CD36 and CD47) onNB4 cells before and after RA treatment. Except for alpha IIb beta 3. which was induced on RA-treated cells, we found no significant increase in the expression of the other receptors, and a decrease in the expression of CD36, upon RX treatment. Preincubation of RX-treated cells with blocking antibodies demonstrated a role for alpha 4 beta 1 and alpha 5 beta 1 in cell adhesion to fibronectin and alpha 5 beta 1, alpha IIb beta 3, CD36 and CD47 in cell adhesion to thrombospondin. Experiments with the synthetic peptides GRGDS(0.2mM) and CSVTCG (0.2 mM) confirmed the participation of integrins, and integrins and CD36, in adhesion of RA-treated cells to fibronectin and thrombospondin, respectively Further inhibition by heparin (10pg/ml) and/or recombinant heparin-binding domain of thrombospondin (TSP18) indicated the additional participation of heparin-like receptors in cell adhesion to thrombospondin. Our results indicate that increase in NB4 cell adhesion to fibronectin and thrombospondin upon RX treatment is Likely to occur through a modulation of the functional state of several receptors for these proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 02:29:53