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Titolo:
Specific targeting of activated endothelium in rat adjuvant arthritis witha Tc-99m-radiolabeled E-selectin-binding peptide
Autore:
Zinn, KR; Chaudhuri, TR; Smyth, CA; Wu, Q; Liu, HG; Fleck, M; Mountz, JD; Mountz, JM;
Indirizzi:
Univ Alabama, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 iv Alabama, Birmingham, AL 35294 USA
Titolo Testata:
ARTHRITIS AND RHEUMATISM
fascicolo: 4, volume: 42, anno: 1999,
pagine: 641 - 649
SICI:
0004-3591(199904)42:4<641:STOAEI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADHESION MOLECULES; RHEUMATOID-ARTHRITIS; MONOCLONAL-ANTIBODY; SYNOVIAL TISSUE; P-SELECTIN; EXPRESSION; RECRUITMENT; PROTEIN; MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Zinn, KR UnivALlabama, Boshell Bldg,BDB-Room 821,Box 11,1808 7th Ave S, Birmingham, Univ Alabama Boshell Bldg,BDB-Room 821,Box 11,1808 7th Ave S Birmingham AL USA 35294
Citazione:
K.R. Zinn et al., "Specific targeting of activated endothelium in rat adjuvant arthritis witha Tc-99m-radiolabeled E-selectin-binding peptide", ARTH RHEUM, 42(4), 1999, pp. 641-649

Abstract

Objective. To determine the potential of an E-selectin-binding peptide (ESbp) to specifically bind activated endothelium in rheumatoid arthritis (RA)animal models. Methods. ESbp (KYDGDITWDQLWDLMK; 2,027 daltons) was labeled with biotin and Tc-99m. The affinity of ESbp derivatives for E-selectin was measured by enzyme-linked immunosorbent assay. The binding of biotin-ESbp was compared with that of an anti-E-selectin antibody, by immunohistochemical analyses ofhuman synovial sections and sections from the Mycoplasma pulmonis MRL-lpr/lpr mouse arthritis model. Tc-99m-ESbp was sequentially imaged in vivo witha gamma camera in the rat adjuvant-induced arthritis model. Results. E-selectin expression was detected in human RA synovium and mousearthritic synovium using biotin-ESbp. Both biotin-ESbp and Tc-99m-labeled ESbp had high affinity for E-selectin (dissociation constant 2-5 nM). In vivo imaging showed specific binding of Tc-99m-ESbp to the rat ankle joint prior to clinical manifestations of inflammation. Conclusion. These results demonstrate that activated endothelium can be targeted with Tc-99m-ESbp. The specificity of targeting can be used to evaluate up-regulation of E-selectin in RA models, and to follow changes in this up-regulation during treatment trials.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/08/20 alle ore 15:09:10