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Titolo:
Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins
Autore:
Cirkovic, TD; Bukilica, MN; Gavrovic, MD; Vujcic, ZM; Petrovic, S; Jankov, RM;
Indirizzi:
Torlak, Inst Virol & Immunbiol, YU-11221 Belgrade, Yugoslavia Torlak Belgrade Yugoslavia YU-11221 nbiol, YU-11221 Belgrade, Yugoslavia Ctr Clin, YU-11000 Belgrade, Yugoslavia Ctr Clin Belgrade Yugoslavia YU-11000 lin, YU-11000 Belgrade, Yugoslavia Fac Chem, YU-11000 Belgrade, Yugoslavia Fac Chem Belgrade Yugoslavia YU-11000 hem, YU-11000 Belgrade, Yugoslavia
Titolo Testata:
ALLERGY
fascicolo: 2, volume: 54, anno: 1999,
pagine: 128 - 134
SICI:
0105-4538(199902)54:2<128:PAICOL>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLACEBO-CONTROLLED IMMUNOTHERAPY; DOUBLE-BLIND; RUSH IMMUNOTHERAPY; EFFICACY; ANTIBODY; SUBCLASS; EXTRACT; SAFETY;
Keywords:
allergoid; chemical modification; Dactylis glomerata; maleic anhydride; orchard grass; skin prick test; succinic anhydride;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Petrovic, S Torlak,lavia Virol & Immunbiol, Vojvode Stepe 458, YU-11221 Belgrade, Yugos Torlak Vojvode Stepe 458 Belgrade Yugoslavia YU-11221 , Yugos
Citazione:
T.D. Cirkovic et al., "Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins", ALLERGY, 54(2), 1999, pp. 128-134

Abstract

Background: Orchard grass (Dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. Chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of Lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins. Methods: Physicochemical characterization of derivatives was done by gel chromatography, SDS-PAGE, and isoelectric focusing. To examine the IgE-binding properties of these derivatives, we carried out immunoblotting. To examine the ability of derivatives to induce IgG production, we immunized rabbits. Skin prick testing with the allergoids was performed on 15 individuals allergic to orchard grass pollens and on two healthy subjects. Results: II was shown that the modified proteins retain their original molecular weights, but change pi to more acidic values. In the case of allergoids, a strong reduction in IgE binding was found. Immunization of rabbits with allergoids showed that the derivatives retain the ability to induce IgGproduction, and that the antisera obtained in such a way react to native (unmodified) extract. The ability of derivatives to induce allergic reactionwas significantly reduced. The patients (86.6%) included in our study exhibited less than 50% of native extract response. Among them, 53.3% had no response to one or both allergoids. Conclusions: These modification procedures yield allergoids with a reducedallergenic activity and preserved immunogenic potential suitable for use in immunotherapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 21:49:52