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Titolo:
SEMICONDUCTOR LASER-INDUCED FLUORESCENCE DETECTION IN CAPILLARY ELECTROPHORESIS USING A CYANINE DYE
Autore:
CHEN FTA; TUSAK A; PENTONEY S; KONRAD K; LEW C; KOH E; STERNBERG J;
Indirizzi:
BECKMAN INSTRUMENTS INC,2500 HARBOR BLVD FULLERTON CA 92634
Titolo Testata:
Journal of chromatography
fascicolo: 2, volume: 652, anno: 1993,
pagine: 355 - 360
Fonte:
ISI
Lingua:
ENG
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
5
Recensione:
Indirizzi per estratti:
Citazione:
F.T.A. Chen et al., "SEMICONDUCTOR LASER-INDUCED FLUORESCENCE DETECTION IN CAPILLARY ELECTROPHORESIS USING A CYANINE DYE", Journal of chromatography, 652(2), 1993, pp. 355-360

Abstract

Cy5, an activated carboxyl cyanine fluorophore, was characterized by capillary electrophoresis (CE) using a semiconductor laser at 652 nm to induce fluorescence. Hydrolysis of the activated Cy5 in the presenceof ammonia results in the formation of a mono- and diamide and a dicarboxylic acid. A Cy5-labeled oligonucleotide M(13) primer for DNA sequencing (M13mp18 template) was synthesized with a purity of better than95%. The labeled primer was analyzed by liquid chromatography, using UV-visible detection, and by CE, monitored by laser-induced fluorescence (LIE) detection. Analysis of the Cy5-labeled oligonucleotide primerby CE-LIF in a 9% polyacrylamide gel-filled capillary indicated the purity of the major Cy5-oligonucleotide primer was greater than 90%. The detection sensitivity for Cy5-based CE-LIF detection system with a 2.5-mW red semiconductor laser is about 10(-10) M.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 16:19:49