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Titolo:
CLONING OF CELL-SPECIFIC SECRETED AND SURFACE-PROTEINS BY SUBTRACTIVEANTIBODY SCREENING
Autore:
SCHERER PE; BICKEL PE; KOTLER M; LODISH HF;
Indirizzi:
YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT CELL BIOL,1300 MORRIS PK AVE BRONX NY 10461 MASSACHUSETTS GEN HOSP,DEPT MED,DIABET UNIT BOSTON MA 02114 HARVARD UNIV,SCH MED BOSTON MA 02114 MIT,DEPT BIOL CAMBRIDGE MA 02139 WHITEHEAD INST BIOMED RES CAMBRIDGE MA 02142
Titolo Testata:
Nature biotechnology
fascicolo: 6, volume: 16, anno: 1998,
pagine: 581 - 586
SICI:
1087-0156(1998)16:6<581:COCSAS>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-DENSITY-LIPOPROTEIN; MONOCLONAL-ANTIBODIES; EXPRESSION CLONING; 3T3-L1 ADIPOCYTES; AMINE OXIDASE; VI COLLAGEN; GLYCOPROTEIN; SEQUENCE; VIGILIN; RAT;
Keywords:
DIFFERENTIAL EXPRESSION; ADIPOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
P.E. Scherer et al., "CLONING OF CELL-SPECIFIC SECRETED AND SURFACE-PROTEINS BY SUBTRACTIVEANTIBODY SCREENING", Nature biotechnology, 16(6), 1998, pp. 581-586

Abstract

To identify and clone genes that encode cell-or tissue-specific secreted and surface proteins, a polyclonal antiserum was raised against a complex mixture of surface or secreted proteins from the target cell, followed by immunodepletion of antibodies that recognize proteins froma nontarget cell or tissue. The depleted antiserum is used to screen bacteriophage cDNA expression libraries. Because of our interest in how adipocytes communicate with other cells, we have used this method toclone cDNAs encoding secreted and plasma membrane proteins that are induced during adipocyte differentiation. We describe several of these,including a novel plasma membrane-associated protein, S3-12.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 01:25:18