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Titolo:
METABOLIC SUPPRESSION IN ANOXIC FROG-MUSCLE
Autore:
WEST TG; BOUTILIER RG;
Indirizzi:
UNIV CAMBRIDGE,DEPT ZOOL,DOWNING ST CAMBRIDGE CB2 3EJ ENGLAND
Titolo Testata:
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
fascicolo: 4, volume: 168, anno: 1998,
pagine: 273 - 280
SICI:
0174-1578(1998)168:4<273:MSIAF>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENERGY-METABOLISM; CRUCIAN CARP; TURTLE BRAIN; ADENOSINE; DEFENSE; HYPOXIA; SYSTEM; SLICES; INSITU;
Keywords:
AMPHIBIAN; ANOXIA; HYPOMETABOLISM MICROCALORIMETRY; MICRODIALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
T.G. West e R.G. Boutilier, "METABOLIC SUPPRESSION IN ANOXIC FROG-MUSCLE", Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 168(4), 1998, pp. 273-280

Abstract

Microcalorimetry is the only direct method for measuring moment-to-moment changes in whole-cell metabolism las heat output) during anoxia. We have adapted this methodology, in conjunction with standard muscle isolation techniques, to monitor metabolic transitions in isolated frog (Rana temporaria) sartorius muscle during anoxia and recovery (reoxygenation). Anoxia (sustained 1 h, following 2 h progressive hypoxia) suppressed muscle heat output to 20% of the stable normoxic level. Thiseffect was fully reversible upon reoxygenation. Metabolite profiles were consistent with other anoxia-tolerant vertebrates - most notably, adenosine triphosphate (ATP) content during anoxia and reoxygenation remained unchanged from normoxia (pre-anoxic control). In addition, theconcentration of K+ ions ([K+] in interstitial dialysates remained stable (2-3 mM) throughout anoxia and recovery. Interstitial [lactate(-)] increased slightly, in accord with anaerobiosis supporting suppressed metabolic rates during anoxia. The degree of anoxic suppression of metabolism observed is similar to other vertebrate models of anoxia tolerance. Furthermore, stable ATP concentrations and interstitial [K+] in the isolated tissue suggests that intrinsic mechanisms suppress metabolism in a manner that coordinates ATP supply and demand and avoids the severe ion imbalances that are characteristic of hypoxia-sensitive systems.

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Documento generato il 04/06/20 alle ore 01:42:04