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Titolo:
FREQUENCIES OF OCCURRENCE OF ALL HUMAN-CHROMOSOMES IN MICRONUCLEI FROM NORMAL AND 5-AZACYTIDINE-TREATED LYMPHOCYTES AS REVEALED BY CHROMOSOME PAINTING
Autore:
FAUTH E; SCHERTHAN H; ZANKL H;
Indirizzi:
UNIV KAISERSLAUTERN,ABT HUMANBIOL & HUMANGENET,POSTFACH 3049 D-67653 KAISERSLAUTERN GERMANY UNIV KAISERSLAUTERN,ABT HUMANBIOL & HUMANGENET D-67653 KAISERSLAUTERNGERMANY
Titolo Testata:
Mutagenesis
fascicolo: 3, volume: 13, anno: 1998,
pagine: 235 - 241
SICI:
0267-8357(1998)13:3<235:FOOOAH>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RADIATION-INDUCED MICRONUCLEI; IN-SITU HYBRIDIZATION; FLUORESCENCE INSITU HYBRIDIZATION; MOUSE BONE-MARROW; SISTER-CHROMATID EXCHANGES; BLOCKED HUMAN-LYMPHOCYTES; CENTROMERIC DNA PROBES; SATELLITE DNA; SPINDLE POISONS; ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
E. Fauth et al., "FREQUENCIES OF OCCURRENCE OF ALL HUMAN-CHROMOSOMES IN MICRONUCLEI FROM NORMAL AND 5-AZACYTIDINE-TREATED LYMPHOCYTES AS REVEALED BY CHROMOSOME PAINTING", Mutagenesis, 13(3), 1998, pp. 235-241

Abstract

Chromosome painting with library DNA probes specific for all human chromosomes was used to study the chromosomal content of micronuclei (MN) in normal and 5-azacytidine (5-aza-C)-treated lymphocyte cultures. More than 60 000 normal lymphocytes were screened for associated MN after in situ hybridization, At least 50 MN were scored for each probe. With the exception of chromosomes 12 and 19, which did not occur in MN,all other chromosomes were detected in MN at frequencies varying from1 to 11.5%. Treatment of lymphocyte cultures with 5-aza-C induced preferential exclusion of chromosomes 1 (34%), 9 (32%) and 16 (20%) material in MN, whereas chromosome 8, 10, 12-15 and 21 material was not detected in MN. The results obtained from normal lymphocytes allow for the first time an estimation of the frequency of occurrence of all chromosomes in spontaneously occurring MN in human cells. Data derived from5-aza-C-treated lymphocytes are furthermore consistent with the view that undermethylation of heterochromatin may be associated with loss of specific chromosomes at metaphase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/08/20 alle ore 20:13:31