Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IMPACT OF STARVATION-REFEEDING ON KINETICS AND PROTEIN EXPRESSION OF TROUT LIVER NADPH-PRODUCTION SYSTEMS
Autore:
BARROSO JB; PERAGON J; CONTRERASJURADO C; GARCIASALGUERO L; CORPAS FJ; ESTEBAN FJ; PEINADO MA; DELAHIGUERA M; LUPIANEZ JA;
Indirizzi:
UNIV GRANADA,CTR CIENCIAS BIOL,DEPT BIOQUIM & BIOL MOL,AVE FUENTENUEVA S-N E-18001 GRANADA SPAIN UNIV JAEN,FAC EXPT SCI,DEPT BIOCHEM & MOL BIOL E-23071 JAEN SPAIN UNIV GRANADA,CTR BIOL SCI,DEPT ANIM BIOL & ECOL E-18001 GRANADA SPAIN CSIC,ESTAC EXPT ZAIDIN,DEPT BIOCHEM CELLULAR & MOL BIOL PLANTS E-18008 GRANADA SPAIN
Titolo Testata:
American journal of physiology. Regulatory, integrative and comparative physiology
fascicolo: 6, volume: 43, anno: 1998,
pagine: 1578 - 1587
SICI:
0363-6119(1998)43:6<1578:IOSOKA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLE MOLECULAR-FORMS; RAINBOW-TROUT; SALMO-GAIRDNERI; MALIC ENZYME; SHUNT DEHYDROGENASES; CARBOHYDRATE DIET; METABOLISM; GLUCOSE-6-PHOSPHATE-DEHYDROGENASE; HEPATOCYTES; TEMPERATURE;
Keywords:
RAINBOW TROUT; LOW-FAT AND HIGH-CARBOHYDRATE DIET; GLUCOSE-6-PHOSPHATE DEHYDROGENASE; 6-PHOSPHOGLUCONATE DEHYDROGENASE; MALIC ENZYME; DIMERIC BANDING PATTERN; IMMUNOHISTOCHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
J.B. Barroso et al., "IMPACT OF STARVATION-REFEEDING ON KINETICS AND PROTEIN EXPRESSION OF TROUT LIVER NADPH-PRODUCTION SYSTEMS", American journal of physiology. Regulatory, integrative and comparative physiology, 43(6), 1998, pp. 1578-1587

Abstract

Herein we report on the kinetic and protein expression of glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase, and malic enzyme (ME) in the liver of the trout (Oncorhynchus mykiss) during a long-term starvation-refeeding cycle. Starvation significantly depressed the activity of these enzymes by almost 60%, without changing the Michaelis constant. The time response to this nutritional stimulusincreased with fish weight. The sharp decline in G6PDH and ME activities was due to a specific protein-repression phenomenon, as demonstrated by molecular and immunohistochemical analyses. Also, the dimeric banding pattern of liver G6PDH shifted from the fully reduced and partially oxidized forms, predominant in control, to a fully oxidized form, more sensitive to proteolytic inactivation. Refeeding caused opposite effects in both protein concentration and enzyme activities of about twice the control values in the first stages, later reaching the normalenzyme activity levels. Additionally, the partially oxidized form of G6PDH increased. The kinetics of these enzymes were examined in relation to the various metabolic roles of NADPH. These results clearly indicate that trout liver undergoes protein repression-induction processesunder these two contrasting nutritional conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 17:20:48