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Titolo:
CASPASE-MEDIATED CLEAVAGE OF THE UBIQUITIN-PROTEIN LIGASE NEDD4 DURING APOPTOSIS
Autore:
HARVEY KF; HARVEY NL; MICHAEL JM; PARASIVAM G; WATERHOUSE N; ALNEMRI ES; WATTERS D; KUMAR S;
Indirizzi:
INST MED & VET SCI,HANSON CTR CANC RES,POB 14,RUNDLE MALL,FROME RD ADELAIDE SA 5000 AUSTRALIA INST MED & VET SCI,HANSON CTR CANC RES ADELAIDE SA 5000 AUSTRALIA UNIV QUEENSLAND,ROYAL BRISBANE HOSP,QUEENSLAND INST MED RES HERSTON QLD 4029 AUSTRALIA THOMAS JEFFERSON UNIV,KIMMEL CANC INST,CTR APOPTOSIS RES PHILADELPHIAPA 19107
Titolo Testata:
The Journal of biological chemistry
fascicolo: 22, volume: 273, anno: 1998,
pagine: 13524 - 13530
SICI:
0021-9258(1998)273:22<13524:CCOTUL>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ICE-LIKE PROTEASES; CELL-DEATH; LAMIN-A; C-MYC; EXPRESSION; SUBSTRATE; ENZYME; IDENTIFICATION; PROTEOLYSIS; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
K.F. Harvey et al., "CASPASE-MEDIATED CLEAVAGE OF THE UBIQUITIN-PROTEIN LIGASE NEDD4 DURING APOPTOSIS", The Journal of biological chemistry, 273(22), 1998, pp. 13524-13530

Abstract

The onset of apoptosis is coupled to the proteolytic activation of a family of cysteine proteases, termed caspases, These proteases cleave their target proteins after an aspartate residue. Following caspase activation during apoptosis, a number of specific proteins have been shown to be cleaved. Here we show that Nedd4, a ubiquitin-protein ligase containing multiple WW domains and a calcium/lipid-binding domain, is also cleaved during apoptosis induced by a variety of stimuli including Fas-ligation, gamma-radiation, tumor necrosis factor-alpha, C-8 ceramide, and etoposide treatment. Extracts from apoptotic cells also generated cleavage patterns similar to that seen in vivo, and this cleavage was inhibited by an inhibitor of caspase-3-like proteases, In vitro,Nedd4 was cleaved by a number of caspases, including caspase-1, -3, -6, and -7. By site-directed mutagenesis, one of the in vitro caspase cleavage sites in mouse Nedd4 was mapped to a DQPD(237 down arrow) sequence, which is conserved between mouse, rat, and human proteins. This is the first report demonstrating that an enzyme of the ubiquitin pathway is cleaved by caspases during apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:08:55