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Titolo:
DEVELOPMENT OF A GENETIC-TRANSFORMATION SYSTEM FOR AN ALGA-LYSING BACTERIUM
Autore:
KATO J; AMIE J; MURATA Y; KURODA A; MITSUTANI A; OHTAKE H;
Indirizzi:
HIROSHIMA UNIV,DEPT FERMENTAT TECHNOL HIROSHIMA 7398527 JAPAN NATL FISHERIES UNIV,DEPT BIOL & AQUACULTURE SHIMONOSEKI YAMAGUCHI 75665 JAPAN
Titolo Testata:
Applied and environmental microbiology
fascicolo: 6, volume: 64, anno: 1998,
pagine: 2061 - 2064
SICI:
0099-2240(1998)64:6<2061:DOAGSF>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLIDING BACTERIUM; CYTOPHAGA SP; MARINE; GROWTH; JAPAN; SEA; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
J. Kato et al., "DEVELOPMENT OF A GENETIC-TRANSFORMATION SYSTEM FOR AN ALGA-LYSING BACTERIUM", Applied and environmental microbiology, 64(6), 1998, pp. 2061-2064

Abstract

Four marine bacteria, Alteromonas sp, strains A27, A28, A29, and A30,that lyse the diatom Skeletonema costatum NIES-324 were isolated fromcoastal seawater samples. They were also able to lyse the diatoms Talassiosira sp, and Eucampia zodiacs and the raphidophycean flagellate Chattonella antiqua, Cryptic indigenous plasmids, designated pAS28 and pAS29, were detected in Alteromonas sp, strains A28 and A29, respectively. These plasmids appeared to be similar based on size and restriction site analysis, A shuttle vector that replicates in Escherichia coliand Alteromonas sp. strain A28 was constructed by fusing pAS28 and E.coli vector pCRIIc. The 16-kbp chimeric plasmid, designated pASS1, had the ability to transform strain A28 at a frequency of 10(6) transformants per mu g of DNA. Deletion analysis of pASS1 showed that the 4.7-kb EcoRI-HindIII region of pAS28 was essential for plasmid maintenancein strain A28, This EcoRI-HindIII fragment contained an open reading frame which appeared to encode a 708-amino-acid protein.

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Documento generato il 21/09/20 alle ore 09:45:37