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Titolo:
QUANTITATIVE-DETERMINATION OF TRICYCLIC ANTIDEPRESSANTS AND THEIR METABOLITES IN PLASMA BY SOLID-PHASE EXTRACTION (BOND-ELUT TCA) AND SEPARATION BY CAPILLARY GAS-CHROMATOGRAPHY WITH NITROGEN-PHOSPHORUS DETECTION
Autore:
DELATORRE R; ORTUNO J; PASCUAL JA; GONZALEZ S; BALLESTA J;
Indirizzi:
UNIV AUTONOMA BARCELONA,INST MUNICIPAL INVEST MED,DEPT PHARMACOL,DRUGRES UNIT,DR ALGUADER 80 E-08003 BARCELONA SPAIN
Titolo Testata:
Therapeutic drug monitoring
fascicolo: 3, volume: 20, anno: 1998,
pagine: 340 - 346
SICI:
0163-4356(1998)20:3<340:QOTAAT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIQUID-CHROMATOGRAPHY; ANTI-DEPRESSANTS; DRUGS; SERUM; AMITRIPTYLINE; NORTRIPTYLINE; METHODOLOGY; RESOLUTION; BLOOD; HPLC;
Keywords:
TRICYCLIC ANTIDEPRESSANTS; GAS CHROMATOGRAPHY; SOLID-PHASE EXTRACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
R. Delatorre et al., "QUANTITATIVE-DETERMINATION OF TRICYCLIC ANTIDEPRESSANTS AND THEIR METABOLITES IN PLASMA BY SOLID-PHASE EXTRACTION (BOND-ELUT TCA) AND SEPARATION BY CAPILLARY GAS-CHROMATOGRAPHY WITH NITROGEN-PHOSPHORUS DETECTION", Therapeutic drug monitoring, 20(3), 1998, pp. 340-346

Abstract

An analytical method for the simultaneous determination of amitriptyline, nortriptyline, imipramine, desipramine, clomipramine, and desmethylclomipramine in human plasma using promazine as internal standard isdescribed. The method is based on a solid-phase extraction procedure using the Bond-Elut TCA columns followed by separation and detection using capillary gas chromatography with a specific nitrogen phosphorousdetector (GC/NPD). Using the new extraction procedure, the problem ofadsorption losses was overcome, and good recoveries were achieved forall compounds tested (>87%). Furthermore, clean extracts free of chromatographic interferences were obtained. Complete separation of underivatized, tricyclic antidepressant compounds was achieved in <11 minutes with reliable chromatographic performance. The Limits of detection ranged from 1.2 to 5.8 mu g/l. Calibration curves, showing goad linearity, were prepared covering the therapeutic concentrations range expected (20 to 500 mu g/l). The interassay precision values (RSD) ranged from 4.5% to 9.8%. It is concluded that extraction of amitriptyline, nortriptyline, imipramine, desipramine, clomipramine, and desmethylclomipramine, with Bond Elut TCA solid-phase columns followed by their detection using GC/NPD provides a sensitive and reproducible method that can be easily automated for immediate and routine analysis of clinical samples.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 19:29:00