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Titolo:
NUCLEAR-ENVELOPE DISASSEMBLY IN MITOTIC EXTRACT REQUIRES FUNCTIONAL NUCLEAR-PORES AND A NUCLEAR LAMINA
Autore:
COLLAS P;
Indirizzi:
NORWEGIAN COLL VET MED,DEPT BIOCHEM,POB 8146 DEP N-0033 OSLO NORWAY
Titolo Testata:
Journal of Cell Science
, volume: 111, anno: 1998,
parte:, 9
pagine: 1293 - 1303
SICI:
0021-9533(1998)111:<1293:NDIMER>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-FREE SYSTEM; B-RECEPTOR; CHROMOSOME CONDENSATION; SPINDLE FORMATION; PROTEIN; MEMBRANE; PHOSPHORYLATION; INVITRO; TRANSPORT; MITOSIS;
Keywords:
NUCLEAR ENVELOPE BREAKDOWN; LAMIN B; LAMIN B RECEPTOR; PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
P. Collas, "NUCLEAR-ENVELOPE DISASSEMBLY IN MITOTIC EXTRACT REQUIRES FUNCTIONAL NUCLEAR-PORES AND A NUCLEAR LAMINA", Journal of Cell Science, 111, 1998, pp. 1293-1303

Abstract

Using sea urchin embryonic and in-vitro-assembled nuclei incubated insea urchin mitotic extract, I provide evidence for a requirement for functional nuclear pores and a nuclear lamina for nuclear envelope disassembly in vitro, In intel phase gastrula nuclei, lamin B interacts with p56, an integral protein of inner nuclear membrane cross-reacting with antibodies to human lamin B receptor. Incubation of gastrula nuclei in mitotic cytosol containing an ATP-generating system rapidly induces hyperphosphorylation of p56 and lamin B, Subsequently, p56-lamin Binteractions are weakened and the two proteins segregate into distinct nuclear envelope-derived vesicles upon disassembly of nuclear membranes and of the lamina. Nuclear disassembly is accompanied by chromatincondensation. Blocking nuclear pore function with wheat germ agglutinin or antibodies to nucleoporins prevents p56 and lamin B hyperphosphorylation, nuclear membrane breakdown and lamina solubilization, These events are not rescued by permeabilization of nuclear membranes to molecules of 150,000 M-r With lysolecithin. In-vitro-assembled nuclei containing nuclear membranes with functional pores but no lamina do not disassemble in mitotic cytosol in spite of p56 hyperphosphorylation. Nuclear import of soluble lamin B and reformation of a lamina in interphase extract restores nuclear disassembly in mitotic cytosol. The data indicate a role for functional nuclear pores in nuclear disassembly invitro. They show that p56 hyperphosphorylation is not sufficient for nuclear membrane disassembly in mitotic cytosol and argue that the nuclear lamina plays a critical role in nuclear disassembly at mitosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:39:59