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Titolo:
CRYOPRESERVATION OF IN-VITRO GROWN MERISTEMS OF STRAWBERRY (FRAGARIA X ANANASSA DUCH.) BY ENCAPSULATION-VITRIFICATION
Autore:
HIRAI D; SHIRAI K; SHIRAI S; SAKAI A;
Indirizzi:
HOKKAIDO PREFECTURAL PLANT GENET RESOURCES CTR,363-2 MINAMI TAKINOKAWA TAKIKAWA 073 JAPAN
Titolo Testata:
Euphytica
fascicolo: 1, volume: 101, anno: 1998,
pagine: 109 - 115
SICI:
0014-2336(1998)101:1<109:COIGMO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
WASABI WASABIA-JAPONICA; TRIFOLIUM-REPENS L; SHOOT-TIPS; APICAL MERISTEMS; PLANT-REGENERATION; INVITRO PLANTLETS; DEHYDRATION; SURVIVAL; CELLS; SINENSIS;
Keywords:
CRYOPRESERVATION; ENCAPSULATION-VITRIFICATION; MERISTEMS; STRAWBERRY; FRAGARIA X ANANASSA DUCH.; VITRIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
D. Hirai et al., "CRYOPRESERVATION OF IN-VITRO GROWN MERISTEMS OF STRAWBERRY (FRAGARIA X ANANASSA DUCH.) BY ENCAPSULATION-VITRIFICATION", Euphytica, 101(1), 1998, pp. 109-115

Abstract

Alginate-coated meristems from in vitro-grown strawberry (Fragaria x ananassa Duch.) were successfully cryopreserved following dehydration by a vitrification solution. Excised meristems from cold-hardened plantlets at 4 degrees C for two weeks in the dark were encapsulated into alginate-gel beads containing a mixture of 2 M glycerol plus 0.4 M sucrose. These encapsulated and cryoprotected meristems were dehydrated with a highly concentrated vitrification solution (designated PVS2) for2 hr at 0 degrees C prior to a plunge into liquid nitrogen. Successfully encapsulated vitrified meristems remained green and then developedshoots within one week after plating without intermediary callus formation. The average rate of shoot formation of encapsulated vitrified meristems amounted to nearly 90%. The cryogenic protocol was successfully applied to four cultivars of strawberry. It was also confirmed thatencapsulated vitrified meristems cooled to -196 degrees C produced higher shoot formation than encapsulated dried meristems. Besides, the recovery growth was much earlier than the latter. The encapsulation-vitrification method is easy to handle and produces high levels of shoot formation. Thus, the protocol promises for cryopreservation of strawberry germplasm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:39:55