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Titolo:
GLYCINE AND GABA RECEPTORS IN THE MAMMALIAN RETINA
Autore:
WASSLE H; KOULEN P; BRANDSTATTER JH; FLETCHER EL; BECKER CM;
Indirizzi:
MAX PLANCK INST HIRNFORSCH,DEUTSCHORDENSTR 46 D-60528 FRANKFURT GERMANY UNIV ERLANGEN NURNBERG,INST BIOCHEM D-8520 ERLANGEN GERMANY
Titolo Testata:
Vision research
fascicolo: 10, volume: 38, anno: 1998,
pagine: 1411 - 1430
SICI:
0042-6989(1998)38:10<1411:GAGRIT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC-ACID; CHOLINERGIC AMACRINE CELLS; SUBUNIT MESSENGER-RNAS; CONE BIPOLAR CELLS; RAT SPINAL-CORD; TRANSMITTER-GATED CURRENTS; TIGER SALAMANDER RETINA; MACAQUE MONKEY RETINA; IN-SITU HYBRIDIZATION; DRUG-BINDING PROFILE;
Keywords:
GLYCINE RECEPTORS; GABA(A) RECEPTORS; SYNAPTIC CLUSTERING; GABA(C) RECEPTORS; OSCILLATOR MUTANT MOUSE; RABBIT RETINA; BIPOLAR CELLS; GANGLION CELL; DIRECTIONAL SELECTIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
170
Recensione:
Indirizzi per estratti:
Citazione:
H. Wassle et al., "GLYCINE AND GABA RECEPTORS IN THE MAMMALIAN RETINA", Vision research, 38(10), 1998, pp. 1411-1430

Abstract

Molecular cloning has introduced an unexpected diversity of neurotransmitter receptors. In this study we review the types, the localizationand possible synaptic function of the inhibitory neurotransmitter receptors in the mammalian retina. Glycine receptors (GlyRs) and their localization in the mammalian retina were analyzed immunocytochemically. Specific antibodies against the al subunit of the GlyR (mAb2b) and against all subunits of the GlyR (mAb4a) were used. Both antibodies produced a punctate immunofluorescence, which was shown by electron microscopy to represent clustering of GlyRs at synaptic sites. Synapses expressing the alpha 1 subunit of the GlyR were found on ganglion tell dendrites and on bipolar cell axons. GlyRs were also investigated in the oscillator mutant mouse. The complete loss of the al subunit was compensated for by an apparent upregulation of the other subunits of the GlyR. GABA(A) receptors (GABA(A)Rs) and their retinal distribution were studied with specific antibodies that recognize the alpha 1, alpha 2, alpha 3, beta 1, beta 2, beta 3, gamma 2 and delta subunits. Most antibodies produced a punctate immunofluorescence in the inner plexiform layer (IPL) which was shown by electron microscopy to represent synaptic clustering of GABA(A)Rs. The density of puncta varied across the IPLand different subunits were found in characteristic strata. This stratification pattern was analyzed with respect to the ramification of cholinergic amacrine cells. Using intracellular injection with Lucifer yellow followed by immunofluorescence, we found that GABA(A)Rs composedof different subunits were expressed by the same ganglion cell, however, they were clustered at different synaptic sites. The distribution of GABA(C) receptors was studied in the mouse and in the rabbit retinausing an antiserum that recognizes the p1, p2 and p3 subunits. GABA(C) receptors were found to be clustered at postsynaptic sites. Most, ifnot all of the synapses were found on rod and cone bipolar axon terminals. In conclusion we find a great diversity of glycine and GABA receptors in the mammalian retina: which might match the plethora of morphological types of amacrine cells. This may also point to subtle differences in synaptic function still to be elucidated. (C) 1998 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 03:12:20