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Titolo:
IDENTIFICATION AND CHARACTERIZATION OF A 29-KILODALTON PROTEIN FROM MYCOBACTERIUM-TUBERCULOSIS CULTURE FILTRATE RECOGNIZED BY MOUSE MEMORY EFFECTOR-CELLS
Autore:
ROSENKRANDS I; RASMUSSEN PB; CARNIO M; JACOBSEN S; THEISEN M; ANDERSEN P;
Indirizzi:
STATENS SERUM INST,DEPT TB IMMUNOL,5 ARTILLERIVEJ DK-2300 COPENHAGEN S DENMARK STATENS SERUM INST,DEPT TB IMMUNOL DK-2300 COPENHAGEN S DENMARK STATENS SERUM INST,DEPT CLIN BIOCHEM DK-2300 COPENHAGEN S DENMARK TECH UNIV DENMARK,DEPT BIOCHEM & NUTR DK-2800 LYNGBY DENMARK TECH UNIV MUNICH,INST MICROBIOL,FML WEIHENSTEPHAN D-8000 MUNICH GERMANY
Titolo Testata:
Infection and immunity
fascicolo: 6, volume: 66, anno: 1998,
pagine: 2728 - 2735
SICI:
0019-9567(1998)66:6<2728:IACOA2>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTECTIVE IMMUNITY; BREVIBACTERIUM LINENS; POLYACRYLAMIDE GELS; MICE; RESPONSES; ANTIGEN; INFECTION; SEQUENCE; ELECTROPHORESIS; VACCINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
I. Rosenkrands et al., "IDENTIFICATION AND CHARACTERIZATION OF A 29-KILODALTON PROTEIN FROM MYCOBACTERIUM-TUBERCULOSIS CULTURE FILTRATE RECOGNIZED BY MOUSE MEMORY EFFECTOR-CELLS", Infection and immunity, 66(6), 1998, pp. 2728-2735

Abstract

Culture filtrate proteins from Mycobacterium tuberculosis induce protective immunity in various animal models of tuberculosis. Two molecular mass regions (6 to 10 kDa and 24 to 36 kDa) of short-term culture filtrate are preferentially recognized by Th1 cells in animal models as well as by patients with minimal disease. In the present study, the 24- to 36-kDa region has been studied, and the T-cell reactivity has been mapped in detail. Monoclonal antibodies were generated, and one monoclonal antibody, HYB 71-2, with reactivity against a 29-kDa antigen located in the highly reactive region below the antigen 85 complex was selected. The 29-kDa antigen (CFP29) was purified from M. tuberculosis short-term culture filtrate by thiophilic adsorption chromatography, anion-exchange chromatography, and gel filtration, In its native form, CFP29 forms a polymer with a high molecular mass. CFP29 was mapped in two-dimensional electrophoresis gels as three distinct spots just below the antigen 85 complex component MPT59, CFP29 is present in both culture filtrate and the membrane fraction from M. tuberculosis, suggesting that this antigen is released from the envelope to culture filtrateduring growth, Determination of the N-terminal amino acid sequence allowed cloning and sequencing of the cfp29 gene. The nucleotide sequence showed 62% identity to the bacteriocin Linocin from Brevibacterium linens, Purified recombinant histidine-tagged CFP29 and native CFP29 had similar T-cell stimulatory properties, and they both elicited the release of high levels of gamma interferon from mouse memory effector cells isolated during the recall of protective immunity to tuberculosis. Interspecies analysis by immunoblotting and PCR demonstrated that CFP29 is widely distributed in mycobacterial species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 04:42:44