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Titolo:
MORPHOLOGICAL ALTERATION OF CULTURED TRACHEOBRONCHIAL EPITHELIAL-CELLS IS ACCOMPANIED BY THE EXPRESSION OF CHEMOKINES, MCP-1 AND CINC GRO, IN RATS/
Autore:
YAMAMOTO T; IYONAGA K; TAKEYA M; SAITA N; SUGA M; ANDO M; TAKAHASHI K;
Indirizzi:
KUMAMOTO UNIV,SCH MED,DEPT PATHOL 2,2-2-1 HONJO KUMAMOTO 8600811 JAPAN KUMAMOTO UNIV,SCH MED,DEPT PATHOL 2 KUMAMOTO 8600811 JAPAN KUMAMOTO UNIV,SCH MED,DEPT INTERNAL MED 1 KUMAMOTO 8600811 JAPAN
Titolo Testata:
International journal of experimental pathology
fascicolo: 2, volume: 79, anno: 1998,
pagine: 81 - 92
SICI:
0959-9673(1998)79:2<81:MAOCTE>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCYTE CHEMOATTRACTANT PROTEIN-1; INDUCED NEUTROPHIL CHEMOATTRACTANT; IDIOPATHIC PULMONARY FIBROSIS; BLOOD MONONUCLEAR LEUKOCYTES; KAPPA-B-ACTIVATION; INTERLEUKIN-8 FAMILY; GROWTH-FACTOR; LUNG INJURY; GENE; INFLAMMATION;
Keywords:
RAT; CHEMOKINE; MCP-1; CINC/GRO; EPITHELIAL CELL; CYTOKERATIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
T. Yamamoto et al., "MORPHOLOGICAL ALTERATION OF CULTURED TRACHEOBRONCHIAL EPITHELIAL-CELLS IS ACCOMPANIED BY THE EXPRESSION OF CHEMOKINES, MCP-1 AND CINC GRO, IN RATS/", International journal of experimental pathology, 79(2), 1998, pp. 81-92

Abstract

Morphologically altered epithelial cells are generally observed in fibrotic lung conditions and have been reported to produce several cytokines. To examine the relationship between morphological changes of thetracheobronchial epithelial cells (TBECs) and their chemokine production, we investigated, (1) the mRNA expression and protein secretion ofmonocyte chemoattractant protein-1 (MCP-1) and cytokine-induced neutrophil chemoattractant/gro (CINC/gro), (2) morphological changes by electron microscopy, and (3) cytokeratin (CK) expression, using a primaryculture system of rat TBECs. The constitutive secretion of MCP-1 in the culture supernatant of TBECs increased in a time-dependent manner, whereas the CINC/gro secretion was not changed. These results were consistent with the chemokines' mRNA expression observed by in situ hybridization. The constitutive secretions of MCP-1 and CINC/gro were inhibited partially but significantly by dexamethasone. With the extension of the culture period, the morphology of the TBECs became flat and spindle in shape, similar to squamous metaplasia, as observed on electronmicroscopy, and with strong expression of CK 14. Sequential staining using immunocytochemistry and in situ hybridization revealed the coexpression of MCP-1 mRNA and CK 14. These data indicate a significant relationship between the morphological squamoid alteration and the constitutive expression of MCP-1 but not of CINC/gro. It is thought that thesquamous metaplasia of TBECs may accompany the alteration of cytokineproduction and play an important role in chronic lung inflammation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 23:42:49