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Titolo:
IN-VITRO SELECTED RNA MOLECULES THAT BIND TO ELONGATION-FACTOR TU
Autore:
HORNUNG V; HOFMANN HP; SPRINZL M;
Indirizzi:
UNIV BAYREUTH,BIOCHEM LAB,POSTFACH 10 12 51 D-95440 BAYREUTH GERMANY UNIV BAYREUTH,BIOCHEM LAB D-95440 BAYREUTH GERMANY DEUTSCH FORSCH GEMEINSCHAFT,GRADUIERTENKOLLEG BIOSYNTH PROT & REGULATIHRER A MUNICH GERMANY
Titolo Testata:
Biochemistry
fascicolo: 20, volume: 37, anno: 1998,
pagine: 7260 - 7267
SICI:
0006-2960(1998)37:20<7260:ISRMTB>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
23S RIBOSOMAL-RNA; AMINOACYL-TRANSFER-RNA; CENTER-DOT-GTP; THERMUS-THERMOPHILUS HB8; ALPHA-SARCIN LOOP; EF-TU; ESCHERICHIA-COLI; TERNARY COMPLEX; EFFECTOR REGION; PROTEIN-BIOSYNTHESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
V. Hornung et al., "IN-VITRO SELECTED RNA MOLECULES THAT BIND TO ELONGATION-FACTOR TU", Biochemistry, 37(20), 1998, pp. 7260-7267

Abstract

RNA molecules which bind to elongation factor Tu from T. thermophiluswere isolated from a pool of ribooligonucleotides with a randomized sequence region. These RNAs interact with elongation factor Tu in both the GTP and the GDP form. A slight preference for the GTP form of the protein was observed. The isolated RNA aptamers compete with each other for a common binding site on elongation factor Tu. This binding siteis different from the binding site for aminoacyl-tRNA or the binding site for elongation factor Ts and is located on domain II of elongation factor Tu. The selected RNAs do not bind to elongation factor G. TheEF-Tu binding RNAs share a short consensus sequence, 5'-ACCGAAG-3', which was also found in the alpha-sarcin domain of T. thermophilus 23S rRNA. The isolated RNAs have a hairpin structure with the 5'-ACCGAAG-3' sequence located in non-base-paired regions. Chemical probing and deletion experiments indicate that the consensus sequence is required for the interaction with elongation factor Tu.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 23:04:19