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Titolo:
SIMULTANEOUS ENZYMATIC-SYNTHESIS OF GLUCONIC ACID AND SORBITOL - CONTINUOUS PROCESS-DEVELOPMENT USING GLUCOSE-FRUCTOSE OXIDOREDUCTASE FROM ZYMOMONAS-MOBILIS
Autore:
SILVAMARTINEZ M; HALTRICH D; NOVALIC S; KULBE KD; NIDETZKY B;
Indirizzi:
UNIV BODENKULTUR WIEN,INST FOOD TECHNOL,DIV BIOCHEM ENGN,MUTHGASSE 18A-1190 VIENNA AUSTRIA UNIV BODENKULTUR WIEN,INST FOOD TECHNOL,DIV BIOCHEM ENGN A-1190 VIENNA AUSTRIA
Titolo Testata:
Applied biochemistry and biotechnology
, volume: 70-2, anno: 1998,
pagine: 863 - 868
SICI:
0273-2289(1998)70-2:<863:SEOGAA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Keywords:
GLUCOSE-FRUCTOSE OXIDOREDUCTASE; SORBITOL; GLUCONIC ACID; ZYMOMONAS MOBILIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
M. Silvamartinez et al., "SIMULTANEOUS ENZYMATIC-SYNTHESIS OF GLUCONIC ACID AND SORBITOL - CONTINUOUS PROCESS-DEVELOPMENT USING GLUCOSE-FRUCTOSE OXIDOREDUCTASE FROM ZYMOMONAS-MOBILIS", Applied biochemistry and biotechnology, 70-2, 1998, pp. 863-868

Abstract

The production of sorbitol and gluconic acid by isolated glucose-fructose oxidoreductase (GFOR) from Zymomonas mobilis has been studied in a convective, 100-mL loop reactor with tangential ultrafiltration Using a dilution rate of 0.04/h and 5 kU/L GFOR, substrate conversion (3 Msugar) in a single stage was >85%, and productivities of 126 g sorbitol/(L.d) were obtained. At a constant recycle rate (3/min) and a membrane area of 50 cm(2), the dilution rates land thus productivities) were however limited by a more than 30-fold reduction of the permeate flow in the presence of high sugar and protein concentrations (5 g/L). Protein was added, together with 10 mM dithiothreitol, to improve the stability of GFOR during substrate turnover and crossflow filtration, thus leading to a stable operation of the enzyme reactor for at least 5 d.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:41:33