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Titolo:
AXOTOMY UP-REGULATES THE ANTEROGRADE TRANSPORT AND EXPRESSION OF BRAIN-DERIVED NEUROTROPHIC FACTOR BY SENSORY NEURONS
Autore:
TONRA JR; CURTIS R; WONG V; CLIFFER KD; PARK JS; TIMMES A; NGUYEN T; LINDSAY RM; ACHESON A; DISTEFANO PS;
Indirizzi:
REGENERON PHARMACEUT INC,777 OLD SAW MILL RIVER RD TARRYTOWN NY 10591
Titolo Testata:
The Journal of neuroscience
fascicolo: 11, volume: 18, anno: 1998,
pagine: 4374 - 4383
SICI:
0270-6474(1998)18:11<4374:AUTATA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; RETROGRADE AXONAL-TRANSPORT; DORSAL-ROOT GANGLION; SPINAL MOTOR-NEURONS; MESSENGER-RNA; SCIATIC-NERVE; SYNAPTIC TRANSMISSION; SYMPATHETIC NEURONS; PERIPHERAL-NERVE; SCHWANN-CELLS;
Keywords:
ANTEROGRADE TRANSPORT; RETROGRADE TRANSPORT; NEUROTROPHIN; BDNF; SCIATIC NERVE; DORSAL ROOT GANGLION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
J.R. Tonra et al., "AXOTOMY UP-REGULATES THE ANTEROGRADE TRANSPORT AND EXPRESSION OF BRAIN-DERIVED NEUROTROPHIC FACTOR BY SENSORY NEURONS", The Journal of neuroscience, 18(11), 1998, pp. 4374-4383

Abstract

In addition to the known retrograde transport of neurotrophins, it isnow evident that endogenous brain-derived neurotrophic factor (BDNF) is transported in the anterograde direction in peripheral and central neurons. We used a double-ligation procedure that distinguishes between anterograde and retrograde flow to quantify the anterograde transport of endogenous neurotrophins and neuropeptides in the peripheral nervous system before and after axotomy. BDNF accumulation proximal to theligation (anterograde transport) was twice that distal to the ligation (retrograde direction). Anterograde transport of nerve growth factorand neurotrophin-3 was not evident. Furthermore, BDNF anterograde transport increased 3.5-fold within 24 hr after sciatic nerve injury or dorsal rhizotomy. Anterograde transport of substance P and calcitonin gene-related peptide decreased after peripheral nerve lesion, demonstrating that there was no generalized increase in anterograde transport. To determine the source of the anterogradely transported BDNF, we performed in situ hybridization in a variety of tissues before and after axotomy. Expression of BDNF mRNA in proximal nerve segments did not change with treatment, showing that the increased accumulation of BDNF was not a result of increased local synthesis. BDNF mRNA and protein were expressed by dorsal root ganglion sensory neurons but not by motor neurons. BDNF mRNA expression was increased 1 d after nerve injury, andBDNF protein was also increased twofold to threefold, suggesting thatsensory neurons are the major contributing source of the increased BDNF traffic in the sciatic nerve. Our results suggest that increased anterogradely transported BDNF plays a role in the early neuronal response to peripheral nerve injury at sites distal to the cell body.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 02:16:02